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Position: Home > Articles > Detection and identification of sweet potato virus diseases in Hubei China Plant Protection 2017 (8) 20-23

湖北甘薯病毒病的检测与鉴定

作  者:
彭小琴;王浩然;张俊;张玮华;郭灵芳;方守国;章松柏
单  位:
长江大学农学院;长江大学化学与环境工程学院
关键词:
甘薯病毒病;危害;RT-PCR;dsRNA;序列分析
摘  要:
2013—2015年采集了湖北黄冈、鄂州、武汉、荆州以及宜昌等5个地区的甘薯病毒病样品,通过双生病毒通用引物PCR扩增、ds RNA技术和序列分析等方法,鉴定了这5个地区甘薯病毒病的病原。结果显示,甘薯羽状斑驳病毒(Sweet potato feathery mottle virus,SPFMV)、甘薯褪绿矮化病毒(Sweet potato chlorotic stunt virus,SPCSV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)和甘薯卷叶病毒(Sweet potato leaf curl Georgia virus,SPLCGV)等4种病毒被检出。其中,SPFMV SPLCGV这两种病毒在湖北皆为首次报道。
译  名:
Detection and identification of sweet potato virus diseases in Hubei
作  者:
Peng Xiaoqin;Wang Haoran;Zhang Jun;Zhang Weihua;Guo Lingfang;Fang Shouguo;Zhang Songbai;College of Agriculture,Yangtze University;College of Chemistry & Environment Engineering,Yangtze University;
关键词:
Sweet potato virus disease;;damage;;RT-PCR;;dsRNA;;sequence analysis
摘  要:
Suspected samples of sweet potato virus diseases were collected from Huanggang, Ezhou, Wuhan, Jingzhou and Yichang in Hubei province from 2013 to 2015, and the pathogens were determined by geminivirus universal primers to amplify, dsRNA technology and other methods. The results showed that 4 viruses were identified as sweet potato feathery mottle virus(SPFMV), sweet potato chlorotic stunt virus(SPCSV), cucumber mosaic virus(CMV) and sweet potato leaf curl Georgia virus(SPLCGV). SPFMV and SPLCGV were firstly reported in Hubei.

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