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当前位置: 首页 > 文章 > 锦灯笼根茎均一多糖P_Ⅰ、P_Ⅱ的制备及其结构研究 吉林农业大学学报 2012,34 (3) 289-293
Position: Home > Articles > Studies on Preparation and Structure of Homogeneous Polysaccharides P_Ⅰ,P_Ⅱ from Rhizome of Physalis alkekengi var. franchetii Journal of Jilin Agricultural University 2012,34 (3) 289-293

锦灯笼根茎均一多糖P_Ⅰ、P_Ⅱ的制备及其结构研究

作  者：
周正辉;焦连庆;于敏;戴宛润;赵成爱
单  位：
中国药科大学;吉林农业大学资源与环境学院;吉林省中医药科学院
关键词：
锦灯笼;根茎;均一多糖;重均分子量;数均分子量;高效液相色谱法
摘  要：
采用水提醇沉法提取锦灯笼根茎中的粗多糖,不同乙醇浓度分级醇沉制得精制多糖,再通过葡聚糖凝胶(Sephadex G-200)色谱法获得2个均一多糖(PⅠ、PⅡ)。采用高效液相色谱法测定均一多糖的纯度及分子量,PMP柱前衍生HPLC法测定单糖组成,红外光谱和13C核磁共振分析均一多糖结构。试验结果表明:PⅠ、PⅡ均为均一性很好的多糖,峰位分子量分别为211 257 D、109 441 D,重均分子量(Mw)分别为330 156 D、172 900 D,数均分子量(Mn)分别为223 934 D、79 693 D,分布宽度(Mw/Mn)分别为1.47 435、2.16 959;PⅠ主要由甘露糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖组成,质量比为1.00∶14.94∶7.22∶48.45∶12.62∶6.96∶3.23,它们的残基都是由β-苷键连接;PⅡ主要由甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成,质量比为1.00∶2.58∶2.22∶7.04∶3.11∶1.35,残基是由β-苷键连接。均一多糖PⅠ、PⅡ为首次从锦灯笼根茎中分离得到的一种新的酸性杂多糖。
译  名：
Studies on Preparation and Structure of Homogeneous Polysaccharides P_Ⅰ,P_Ⅱ from Rhizome of Physalis alkekengi var. franchetii
作  者：
ZHOU Zheng-hui1,2,JIAO Lian-qing1,YU Min1,DAI Wan-run3,ZHAO Cheng-ai2 1.Jilin Academy of Traditional Chinese Medicine,Changchun 130012,China;2 College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China;3.China Pharmaceutical University,Nanjing 210009,China
关键词：
Physalis alkekengi var.franchetii;rhizome;homogeneouspoly saccharide;weight-average molecular weight;number-average molecular weight;HPLC
摘  要：
To extract polysaccharides from rhizome of Physalis alkekengi L var.franchetii,using water extraction and alcohol precipitation method,polysaccharide was refined with different concentrations of ethanol and alcohol precipitation classification system,and two homogeneous polysaccharides(PⅠ,PⅡ)were further obtained with Sephadex(Sephadex G-200) chromatography.Homogeneous polysaccharide purity and molecular weight were determined using high performance liquid chromatography;Monosaccharide composition and structure were tested using pre-column derivatization HPLC method,IR and 13C NMR.The results indicate that both PⅠ and PⅡwere of good homogeneous polysaccharides and their peak molecular weight was 211 257 D and 109 441 D respectively,the Weight-A.M.W(Mw) was 330 156 D and 172 900 D,the Number-A.M.W(Mn) was 223 934 D and 79 693 D,and the distribution width(Mw/Mn) was 1.47 435 and 2.16 959 respectively;Homogeneous polysaccharide PⅠwas composed of mannose,glucuronic acid,galacturonic acid,glucose,galactose,xylose and arabinose(1.00∶14.94∶7.22∶48.45∶12.62∶6.96∶3.23);Homogeneous polysaccharide PⅡwas composed of mannose,rhamnose,glucuronic acid,glucose,galactose and arabinose(1.00∶2.58∶2.22∶7.04∶3.11∶1.35).Residues of homogeneous polysaccharides(PⅠ,PⅡ) were connected by β-glucoside bond.Homogeneous polysaccharides(PⅠ,PⅡ)which are heteropolysaccharides were first isolated from rhizome of Physalis alkekengi var.franchetii.

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