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当前位置: 首页 > 文章 > 农杆菌介导的绿木霉ZBS6的T-DNA转化研究 河南农业科学 2011,40 (12) 106-109
Position: Home > Articles > Agrobacterium-mediated T-DNA Transformation of Trichoderma viride ZBS6 Journal of Henan Agricultural Sciences 2011,40 (12) 106-109

农杆菌介导的绿木霉ZBS6的T-DNA转化研究

作  者：
孙虎;施艳;薛保国;杨丽荣;梁慎;全鑫;刘德畅
单  位：
河南省农业科学院植物保护研究所/河南省农作物病虫害防治重点实验室;河南农业大学植物保护学院
关键词：
绿木霉;T-DNA转化;农杆菌;转化子;生防作用
摘  要：
应用农杆菌介导的T-DNA转化技术,成功转化了生防绿木霉(Trichoderma viride)ZBS6,共获得368个转化子,转化效率为每106个分生孢子产生92个转化子。PCR和Southern blot分子鉴定以及遗传稳定性测定结果表明:转化子T-DNA插入率为90%,均能够稳定遗传,且T-DNA单拷贝插入率达到80%。进一步以小麦全蚀病菌(Gaeumannomyces gramims var.tritici)作为供试病原菌进行室内生防作用测定,最终筛选出2个生防作用显著增强的转化子,分别为T79和T124。农杆菌介导的T-DNA转化技术在绿木霉ZBS6中的成功应用,将为木霉菌功能基因组学研究奠定基础,并为其生防作用分子机制研究提供充足的材料和手段。
译  名：
Agrobacterium-mediated T-DNA Transformation of Trichoderma viride ZBS6
作  者：
SUN Hu1,SHI Yan2,XUE Bao-guo1,YANG Li-rong1,LIANG Shen1,QUAN Xin1,LIU De-chang1(1.Institute of Plant Protection/Key Laboratory of Crop Diseases and Insect Pests Prevention in Henan,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China)
关键词：
Trichoderma viride;T-DNA transformation;Agrobacterium;Transformants;Biological control
摘  要：
In this study,Trichoderma viride ZBS6 was transformed based on agrobacterium-mediated T-DNA transformation technology and 368 transformants were obtained.The transformation efficiency was 92 transformants per 106 spores.The transformants were identified using PCR and Southern blot as well as genetic stability assay.The results showed that the percentage of positive transformants which inherited stably achieved as high as 90% and among them 80% were single-copy transformants.Further indoor biocontrol assay was conducted with Gaeumannomyces gramims var.tritici as test pathogen.Two transformants,T79 and T124,which showed obviously increased antagonism,were screened.This study laid the foundation for functional genomics study of T.viride and also supplied enough materials for insights into the molecular mechanism of antagonism.

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