Genetic Diversity Analysis of Triticum aestivum ssp.yunnanense King Using SSR Makers

GOU Yu-hong1,2,YU Ya-xiong1,LIU Li1,YANG Jin-hua1,CHENG Jia-sheng1,HU Yin-xing1,CHENG Geng1(1.Food Crops Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China;2.College of Agronomy and Biotechnology,Yunnan Agricultural University,Yunnan kunming 650201,China)

Triticum aestivum ssp.yunnanense King;SSR markers;Genetic diversity;Cluster analysis

Triticum aestivum ssp.yunnanense King,which is unique germplasm from Yunnan,was considered as useful germplasm for enhancing genetic diversity of bread wheat,20 SSR markers were used to analyze genetic diversity among 36 cultiars.Totally,39 allelic variations were detected among 36 germplasms,1-3 allelic variations were detected at different SSR locus,with the average of 1.95 alleles per SSR marker.The index of Nei's(1973) gene diversity(H) was 0.00-0.50,with the average of 0.24,Shannon's Information index(I)was 0.00-0.69,with the average of 0.38,Genetic distance(GD) was 0.03-0.62 with the average of 0.24.There were less allelic variations in Triticum aestivum ssp.yunnanense King.The clustering analysis of amplifying DNA bands showed that 36 cultivars were divided into 4 clustering groups at the genetic distance of 0.44 and the genetic distance was shorter between Tiekemai from Tengchong and Dahetouxiaomai from Longling,Dinglangtiekemai from Zhenkang and Duanmangtiekemai from Yongde as well as between Tiekemai 2 from Shuangjiang and Yingkemai 1 from Fengqing,whereas that between Tiekemai-4 from Shuangjiang and others was longer.