Effect of Storage Temperature on the Seed Viability,Alpha Amylase Activity and Its Gene Expression at Germination Stage of Lincang Hulled Wheat

ZHOU Guoyan;LONG Wenjie;LEI Yongtao;CHEN Dan;CAI Qing;WU Shaoyun;Biotechnology and Germplasm Resources Institute of Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Lab of Agricultural Biotechnology,Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture;

Hulled wheat;;Storage temperature;;Alpha amylase activity;;Gene expression

In order to study the relationships amongα-amylase activity,relative expression quantitation(REQ)of their coding genes,storage temperature,storage time and seed vitality indices of Lincang hulled wheat(Triticum aestivumssp.yunnanense)at germination stage,the methods of 3,5-Dinitrosalicylic acid(3,5-DNSA)and real time polymerase chain reaction(RT-PCR)were employed to measureα-amylase activity and REQ of their coding genes,respectively.The results showed that compared with storage at low temperature,theα-amylase activity and REQ of amy1 gene in seeds stored at high temperature were significantly increased.However,REQ of amy3 gene was obviously decreased and had a significant positive correlation withα-amylase activity,and both REQ of amy3 gene andα-amylase activity had a significant positive correlation with seeds viability indices,such as germination rate,germination index,germination potential,germination rate and vigor index.There was a significant negative correlation betweenα-amylase activity and REQ of amy3 gene in seeds stored at low tempera-tures.The results mainly showed that seed storage temperature not only strongly affectedα-amylase activity and REQ of its coding gene,but also greatly altered correlation with seed viability indices.