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当前位置: 首页 > 文章 > ITRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation 农业科学学报 (英文) 2015,14 (7)
Position: Home > Articles > ITRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation Journal of Integrative Agriculture 2015,14 (7)

ITRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

作  者：
Ma Lu;Bu Deng-pan;Yang Yong-xing;Yan Su-mei;Wang Jia-qi
单  位：
Inner Mongolia Agr Univ, Hohhot 010018, Peoples R China;Chinese Acad Agr Sci, Inst Anim Sci, CAAS ICRAF Joint Lab Agroforestry & Sustainable A, State Key Lab Anim Nutr, Beijing 100193, Peoples R China;Northeast Agr Univ, Synerget Innovat Ctr Food Safety & Nutr, Harbin 150030, Peoples R China
关键词：
dairy cow;periparturient;plasma;proteomic;quantitative analysis
摘  要：
Dairy cows undergo tremendous changes in physiological, metabolism and the immune function from pregnancy to lactation that are associated with cows being susceptible to metabolic and infectious diseases. The objective of this study is to investigate the changes of plasma proteome on 21 d before expected calving and 1 d after calving from dairy cows using an integrated proteomic approach consisting of minor abundance protein enrichment by ProteoMiner beads, protein labeling by isobaric tags for relative and absolute quantification, and protein identification by liquid chromatography coupled with tandem mass spectrometry. Nineteen proteins were changed around the time of calving. These proteins were associated with response to stress, including acute-phase response and defense response, based on the proteins annotation. In particular, three up-regulated proteins after calving including factor V, alpha 2-antiplasmin and prothronnbin were assigned into the complement and coagulation pathway. These results may provide new information in elucidating host response to lactation and parturition stress, and inflammatory-like conditions at the protein level. Differential proteins may serve as potential markers to regulate the lactation and parturition stress in periparturient dairy cows.
作  者：
MA Lu;BU Deng-pan;YANG Yong-xing;YAN Su-mei;WANG Jia-qi;Inner Mongolia Agricultural University;State Key Laboratory of Animal Nutrition/CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences;Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University;
关键词：
dairy cow,periparturient,plasma,proteomic,quantitative analysis
摘  要：
Dairy cows undergo tremendous changes in physiological, metabolism and the immune function from pregnancy to lactation that are associated with cows being susceptible to metabolic and infectious diseases. The objective of this study is to investigate the changes of plasma proteome on 21 d before expected calving and 1 d after calving from dairy cows using an integrated proteomic approach consisting of minor abundance protein enrichment by Proteo Miner beads, protein labeling by isobaric tags for relative and absolute quantification, and protein identification by liquid chromatography coupled with tandem mass spectrometry. Nineteen proteins were changed around the time of calving. These proteins were associated with response to stress, including acute-phase response and defense response, based on the proteins annotation. In particular, three up-regulated proteins after calving including factor V, α2-antiplasmin and prothrombin were assigned into the complement and coagulation pathway. These results may provide new information in elucidating host response to lactation and parturition stress, and inflammatory-like conditions at the protein level. Differential proteins may serve as potential markers to regulate the lactation and parturition stress in periparturient dairy cows.

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