当前位置: 首页 > 文章 > 一种适合杨树叶片的蛋白质提取方法 南京林业大学学报(自然科学版) 2007,31 (3) 119-121
Position: Home > Articles > A Method Suitable for Protein Extraction of Poplar Leaves Journal of Nanjing Forestry University(Natural Sciences Edition) 2007,31 (3) 119-121

一种适合杨树叶片的蛋白质提取方法

作  者:
袁坤;王明庥;黄敏仁
单  位:
国家林业局江苏省林木遗传和基因工程重点实验室(南京林业大学)
关键词:
杨树叶片;蛋白质提取;双向电泳
摘  要:
高质量的蛋白样品制备是进行双向电泳的前提条件。杨树叶片中因含有丰富的多酚、色素、多糖等物质,给蛋白质的提取带来了困难。为了找到一种适合杨树叶片的蛋白质提取方法,以南林895杨为实验材料,采用三氯乙酸-丙酮沉淀法对杨树叶片蛋白质进行提取。对所提取的蛋白分别用考马斯亮蓝染色法和银染法进行检测,得到了良好的双向电泳图谱。
译  名:
A Method Suitable for Protein Extraction of Poplar Leaves
作  者:
YUAN Kun,WANG Ming-xiu,HUANG Min-ren*(The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province(Nanjing Forestry University), Nanjing 210037,China)
关键词:
Poplar leaves;Protein extraction;Two-dimensional gel electrophoresis
摘  要:
High-quality protein sample preparation is the premise of two-dimensional gel electrophoresis(2-DE).Poplar leaves contain abundant interfering compounds such as polyphenols,pigments,polysaccharides and so on,which make it difficult to extract proteins.In order to find a method suitable for protein extraction of poplar leaves,the poplar of NL895 was used as the experimental material.The method of trichloroacetic acid(TCA)-acetone was adopted to extract proteins.Coomassie brilliant blue(CBB) and silver staining were used respectively to detect the extracted proteins.Good protein patterns were obtained.This paper provides a basis for poplar proteomics research in our lab.

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