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当前位置: 首页 > 文章 > 适用于双向电泳的杨树叶片蛋白质提取方法的研究 植物研究 2016,36 (3) 469-475
Position: Home > Articles > Optional Protein Extracting Methods of Poplar Leaves for Two-dimensional Gel Electrophoresis Bulletin of Botanical Research 2016,36 (3) 469-475

适用于双向电泳的杨树叶片蛋白质提取方法的研究

作  者：
严冬;刘殿昆;司冬晶;郑密;赵曦阳;曲冠证;李莹
单  位：
东北林业大学林木遗传育种国家重点实验室
关键词：
杨树叶片;Tris-三氯乙酸法,蛋白质提取;双向凝胶电泳
摘  要：
为了获得分离效果好、清晰度高的杨树叶片蛋白质双向电泳图像,本研究以小黑杨、毛果杨和84K杨叶片为材料,采用三氯乙酸—丙酮沉淀法、Tris-饱和酚法和Tris-三氯乙酸法提取杨树叶片总蛋白质,分别采用聚丙烯酰胺凝胶电泳和双向凝胶电泳对蛋白质进行分离,应用Image Master 2D Platinum 6.0软件对这3种蛋白质提取方法得到的双向电泳凝胶进行分析。结果表明:Tris-三氯乙酸法最适用于双向电泳的杨树叶片蛋白质的提取。利用Tris-三氯乙酸法提取蛋白质得到的鲜样中总蛋白质平均含量为949.46μg·g-1,得到的蛋白质点平均数量为567个,所得到的双向电泳凝胶图谱分离效果好、清晰度高。
译  名：
Optional Protein Extracting Methods of Poplar Leaves for Two-dimensional Gel Electrophoresis
作  者：
YAN Dong;LIU Dian-Kun;SI Dong-Jing;ZHENG Mi;ZHAO Xi-Yang;QU Guan-Zheng;LI Ying;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Northeast Forestry University;
关键词：
poplar leaves;;protein extraction;;Tris / TCA method;;two-dimensional gel electrophoresis
摘  要：
In order to obtain the two-dimensional gel electrophoresis images with better separation and definition of poplar leaves,the Populus X,P. trichocarpa Torr. & Gray and P. alba × P. glandulosa were selected to study the optional extracting methods of poplar leaves for two-dimensional gel electrophoresis by using TCA / acetone precipitation,Tris / saturated phenol and Tris / TCA method,respectively. The proteins of poplar leaves were separated by SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis respectively and the images of two-dimensional gel electrophoresis were analyzed by Image Master 2D Platinum 6. 0. Tris / TCA method was more appropriate for the protein extraction of poplar leaves for two-dimensional gel electrophoresis.The average protein content of the fresh sample extracted by Tris / TCA method was 949. 46 μg · g- 1and the average quantity of protein spots was 567 in the two-dimensional gel electrophoresis images which had better separation and definition.

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