作 者：
闫海霞;蒋月喜;王晓国;何荆洲;黄昌艳;邓杰玲;卜朝阳
摘 要：
通过蝶豆种子无菌萌发,筛选出各培养阶段的适宜培养基,为建立蝶豆的组织培养与快繁技术提供参考。结果表明:采用75%酒精表面灭菌30 s结合0.1%的HgCl2表面灭菌10 min的方法,蝶豆的种子无菌萌发时污染率为0,发芽率为36.67%;下胚轴、上胚轴、子叶的愈伤诱导率为100.00%,愈伤分化率分别为82.11%、20.00%、0;愈伤分化的最佳培养基为MS+6-BA0.10mg/L+NAA 0.02 mg/L;上胚轴和下胚轴不能直接诱导出不定芽,子叶可以直接诱导出不定芽;不定芽增殖的适宜培养基为MS+6-BA 1.00 mg/L+IBA 0.50 mg/L;生根适宜培养基为MS+IBA 0.30 mg/L或者1/2 MS+IBA 0.30 mg/L。
译 名：
Study on Tissue Culture and Rapid Propagation of Clitoriaternatea L.
作 者：
YAN Hai-xia;JIANG Yue-xi;WANG Xiao-guo;HE Jing-zhou;HUANG Chang-yan;DENG Jie-ling;BU Zhao-yang;Flower Research Institute,Guangxi Academy of Agricultural Sciences;
关键词：
Clitoriaternatea L.;;Axenic germination;;Callus;;Adventitious bud
摘 要：
The suitable medium for varied stages was screened out by axenic geimination of Clitoriatematea L.to provide references for tissue culture and rapid regeneration of Clitoriatematea L.The results showed that:by the method of 75%ethanol for 30 s +0.1%HgCl_2 for 10 min,the contamination rate was 0 and the germination rate was 36.67%when the seeds were aseptic budding.The callus induction rate of hypocotyls,epicotyls,and cotyledon was 100.00%and the rate of callus differentiation was 82.11%,20.00%and 0,respectively.The optimizing medium for callus differentiation was MS +6-BA 0.10 mg/L + NAA 0.02 mg/L.Adventitious buds cannot be induced directly from epicotyl and hypocotyl,but can be induced directly from cotyledon.The optimizing medium for adventitious bud multiplication is MS +6-BA 1.00 mg/L + IBA 0.SO mg/L.The most suitable medium for adventitious root is MS + IBA0.30 mg/L or 1/2MS + IBA0.30 mg/L.
