单 位：
Department of Bioengineering,Zhuhai Campus of Zunyi Medical College,Zhuhai Guangdong 519041,China;Department of Bioengineering,Zhuhai Campus of Zunyi Medical College,Zhuhai Guangdong 519041,China)
摘 要：
[Objective] To establish a method to determine the content of protocatechuic acid in CORTEX ACAN THOPANACIS.[Method] Content of protocatechuic acid in CORTEX ACAN THOPANACIS was determined by high performance liquid chromatography-four channel UV-Vis detection method.Chromatographic conditions were as follows:ODSC18 chromatographic column(250 mm×4.6 mm,5 μm) ,and buffer solution of methanol and sodium dihydrogen phosphate as the mobile phase(0.1 mol/L,15∶85,V/V,pH=2.5) .4 detection wavelengths were 245,260,275 and 290 nm with the quantitative detection wavelength of 260 nm.[Result] Protocatechuic acid had good linear relationship within the range of 0.5-6.0 mg/L(r=0.999 9) .The average recovery rate was 99.87% with RSD=2.11%.[Conclusion] The method was reliable,stable,and simple,and could be used for the content determination of protocatechuic acid in CORTEX ACAN THOPANACIS.
