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Position: Home > Articles > Isolation and Purification of Tobacco Wildfire Bacteria Using PCR Technique Acta Agriculturae Boreali-Sinica 2015,30 (1) 329-332

应用PCR技术分离纯化烟草野火病菌

作  者:
张萌;万秀清;乔婵;李若;李秀兰;孙剑萍;郭兆奎
单  位:
牡丹江师范学院;牡丹江烟草科学研究所;哈尔滨烟叶公司集贤分公司
关键词:
PCR方法;烟草野火病;分离;纯化
摘  要:
针对烟草野火病原菌的鉴定和分离纯化,设计合成其特异PCR鉴定引物,应用PCR技术对分离的烟草野火病病原细菌进行了纯化分离,并通过接种鉴定方法对纯化分离的病原菌进行了致病性评价,结果表明,经PCR鉴定的阳性和阴性细菌与接种鉴定试验结果一致。从而证实了烟草野火病菌特异PCR鉴定引物及应用PCR技术进行野火病病原细菌分离纯化方法的实用性和可靠性。
译  名:
Isolation and Purification of Tobacco Wildfire Bacteria Using PCR Technique
作  者:
ZHANG Meng;WAN Xiu-qing;QIAO Chan;LI Ruo;LI Xiu-lan;SUN Jian-ping;GUO Zhao-kui;Mudanjiang Normal University;Tobacco Research Institute of Mudanjiang;Jixian Branch of Harbin Tobacco Leaf Corporation;
关键词:
Polymerase chain reaction(PCR);;Tobacco wildfire disease;;Separation;;Purification
摘  要:
Specific PCR identification primers were designed in this study to purify collected Pseudomonas syringae pv. Tabaci isolates using PCR technique. The pathogenicity of purified Pseudomonas syringae pv. Tabaci isolates were evaluated by inoculating tobacco plants. The obtained results verified the specificity of identification PCR primers and proved the practicability and reliability of the isolation and purification of Pseudomonas syringae pv.Tabaci using PCR technique.

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