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当前位置: 首页 > 文章 > Frizzled 3、Frizzled 4基因对黑线仓鼠A:CHA白化性状产生影响的初步分析 黑龙江畜牧兽医 2017 (9) 222-224
Position: Home > Articles > Analysis of the function of Frizzled 3、Frizzled 4 genes in the albino phenotype of Cricetulusgriseus Heilongjiang Animal Science and Veterinary Medicine 2017 (9) 222-224

Frizzled 3、Frizzled 4基因对黑线仓鼠A:CHA白化性状产生影响的初步分析

作  者：
王新;刘慧芳;易思萌;殷慧慧;孙兆增
单  位：
青岛农业大学;军事医学科学院实验动物中心;临沂市农业学校
关键词：
黑线仓鼠;白化突变系(A:CHA);Frizzled 3基因;Frizzled 4基因;实时荧光定量PCR
摘  要：
为了比较Wnt通路重要结合受体Frizzled家族的Frizzled 3、Frizzled 4受体蛋白基因在黑线仓鼠及其白化突变系(A:CHA)皮肤组织的表达差异,分析这两种基因对白化性状产生的影响,试验提取黑线仓鼠和A:CHA的皮肤组织总RNA,利用普通PCR方法筛选引物,通过实时荧光定量PCR技术比较黑线仓鼠和A:CHA皮肤组织Frizzled 3、Frizzled 4基因表达量的差异。结果表明:黑线仓鼠皮肤中的Frizzled 3、Frizzled 4基因mRNA的相对表达量分别是A:CHA组织中的4倍、5倍。Frizzled 3、Frizzled 4基因在A:CHA皮肤组织中的表达量下调。说明Frizzled 3、Frizzled 4基因与A:CHA白化性状的产生存在一定关联。
译  名：
Analysis of the function of Frizzled 3、Frizzled 4 genes in the albino phenotype of Cricetulusgriseus
作  者：
WANG Xin;LIU Huifang;YI Simeng;YIN Huihui;SUN Zhaozeng;Qingdao Agriculture University;Linyi Agriculture School;Laboratory Animal Center of the Academy of Military Medical Science;
关键词：
Cricetulusgriseus;;A:CHA;;Frizzled 3;;Frizzled 4;;Real-time PCR
摘  要：
In the current study,investigation and analysis of the expression level of Frizzled 3,Frizzled 4 of animportantreceptor of Wnt protein between Cricetulusgriseus and A: CHA and the functionon the albino phenotype were carried out. The total mRNA of skin tissue in the Cricetulusgriseus and A: CHA was extracted,and the expression levels of Frizzled 3 and Frizzled 4 were analyzed by using real time PCR. The results showed that the ratio of expression level of Frizzled 3,Frizzled 4 between Cricetulusgriseus and A: CHA were revealed to be increased by 4 and5 times. These data demonstrated that the gene expression level of Frizzled 3,Frizzled 4 in the A: CHA were lower than that in the Cricetulusgriseus. The resulting data suggests the notion the two genes may be contribute to the Albino phenotype of A: CHA.

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