当前位置: 首页 > 文章 > 棉铃虫核型多角体病毒orf80多克隆抗体的制备 西北农业学报 2012,21 (4) 193-196
Position: Home > Articles > Preparation of Polyclonal Antibody of Helicoverpa armigera Single-nucleopolyhedrovirus orf80 Acta Agriculturae Boreali-occidentalis Sinica 2012,21 (4) 193-196

棉铃虫核型多角体病毒orf80多克隆抗体的制备

作  者:
徐利;吕婷婷;甘恩宇;高秋荣;王敦
单  位:
西北农林科技大学生命科学学院;西北农林科技大学植保资源与利用教育部重点实验室
关键词:
棉铃虫核型多角体病毒;orf80;多克隆抗体;ELISA
摘  要:
从HearNPV G4株基因组中克隆得到orf80基因的全序列,将该基因构建于pMAL-c4E载体并在TB1中融合表达,orf80融合表达蛋白分子质量为73.3ku,与预测的蛋白一致,Western blot验证正确。纯化融合蛋白后制备抗原并注射新西兰大白兔从而得到orf80的多克隆抗体,经ELISA检测其效价达到2.56×105,表明该抗体具有较高的免疫原性,可用于orf80编码蛋白的检测及蛋白互作等相关研究。
译  名:
Preparation of Polyclonal Antibody of Helicoverpa armigera Single-nucleopolyhedrovirus orf80
作  者:
XU Li1,2,Lü Tingting1,GAN Enyu1,2,GAO Qiurong2 and WANG Dun2(1.College of Biology Science & Technology,Northwest A&F University,Yangling Shaanxi 712100,China;2.Ministry Education Key Laboratory of Plant Protection Resources & Pest Management,Northwest A&F University,Yangling Shaanxi 712100,China)
关键词:
HearNPV;orf80;Polyclonal antibody;ELISA
摘  要:
The HearNPV orf80 was cloned from Helicoverpa armigera Single-nucleopolyhedrovirus(HearNPV) G4 genome by PCR and the prokaryotic expression vector pMAL-c4E-Ha80 was constructed.The recombinant plasmid was transformed into the Escherichi coli TB1 to express the MBP-Ha80 fushion protein.Purified MBP-Ha80 protein was injected into rabbits as antigen to prepare the polyclonal antibodies of orf80.The titer of anti-HearNPV orf80 polyclonal antibodies was 2.56×105 detected by ELISA.The prepared antibodies can be used to determine orf80 coding protein in the research of protein detection and protein interaction.

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