中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 春兰离体根状茎辐射及EMS诱变方法建立 北方园艺 2009 (1) 59-62
Position: Home > Articles > The Method for Radiation and EMS Induced Mutationon Cymbidium goeringii Rhizomes in Vitro Northern Horticulture 2009 (1) 59-62

春兰离体根状茎辐射及EMS诱变方法建立

作  者：
褚云霞;张永春;靖相密
单  位：
上海市农业科学院园艺所;上海市设施园艺技术重点实验室;上海市农业科学院花卉研究中心
关键词：
春兰;根状茎;60Co;EMS
摘  要：
以野生春兰茎尖培养所得根状茎为材料,用102、03、04、0 Gy60Coγ-射线进行辐照,以不经辐照为对照。处理材料培养在1/2MS+NAA 5 mg/L上进行增殖,经30 d后统计重量及生长点数,同时转入1/2MS+NAA 0.2 mg/L+BA 3 mg/L进行分化培养,经45 d后统计分化出的苗数。结果表明:随着辐照剂量增大,根状茎的增殖特别是新生长点的形成受抑制,苗分化均略有促进,但未达显著水平,高剂量(>30 Gy)将严重抑制增殖与分化,20 Gy应为春兰根状茎辐照诱变的最适剂量。EMS处理分别以0.6%、0.8%浸泡6 h和8 h,以磷酸缓冲液浸泡为对照,结果表明,处理与对照差异显著,随着浓度的增大,根状茎的死亡率明显提高,0.8%浸泡6 h与8 h之间差异不显著。
译  名：
The Method for Radiation and EMS Induced Mutationon Cymbidium goeringii Rhizomes in Vitro
作  者：
CHU Yun-xia1,2,3,ZHANG Yong-chun2,3,4,5,JING Xiang-mi3,4(1.Horticultural Research Institute,Shanghai Academy of Agricultural Science,Shanghai 201106,China;2.Flower Reasearch Institute,Shanghai Academy of Agricultural Science,Shanghai,201106,China;3.Shanghai Key Lab of Protected Horticutural Technology,Shanghai 201106,China;4.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China;5.Forestry and Fruit Research Institute,Shanghai Academy of Agricultural Science,Shanghai 201106,China)
关键词：
Cymbidium goeringii;Rhizomes;60Coγ-Ray;Ethyl methanesulfonate
摘  要：
60Coγ-Ray radiated on rhizomes of Cymbidium goeringii at four dose,there were 10,20,30,40 Gy.The rhizomes were proliferated on 1/2MS+NAA5 mg/L for a month.Then they were transferred to differentiation media,1/2 MS+NAA 0.2 mg/L+BA 3 mg/L.After 45 days,the weight of rhizomes and buds' number were investigated.The results showed that low dose of60Coγ-Ray(<10 Gy)can promote the multiplication and differentiation,but its affect was not salience,high dose(>30 Gy) would restrain the multiplication and differentiation severity on the other hand.The optimum treatment dose for breading was 20 Gy.On the other hand,rhizomes were dipped in 0.6%,0.8% ethyl methanesulfonate(EMS) solution for 6 or 8 hours,then there were transferred to medium.The results were compared with it of phosphate buffer solution.It revealed that EMS could restrain its growth speed,and the mortality was raising with the increasing of EMS concentration.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46