中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > BDNF与TrkB蛋白在小鼠子宫和着床前胚胎中表达规律的研究 黑龙江畜牧兽医 2018 (7) 9-11+16+245-246
Position: Home > Articles > The expression pattern of BDNF and TrkB in mouse uteri and the preimplantation embryos Heilongjiang Animal Science and Veterinary Medicine 2018 (7) 9-11+16+245-246

BDNF与TrkB蛋白在小鼠子宫和着床前胚胎中表达规律的研究

作  者：
刘犇
单  位：
宜春学院生命科学与资源环境学院
关键词：
小鼠;BDNF;TrkB;子宫;着床前胚胎;免疫组织化学
摘  要：
为了研究BDNF与TrkB蛋白在小鼠发情周期和妊娠早期子宫、输卵管及着床前胚胎中的表达规律,试验采用免疫组织化学方法检测BDNF与TrkB蛋白在子宫和输卵管中的表达,采用免疫荧光技术检测着床前不同发育阶段胚胎中蛋白的表达,并利用图像分析软件对两种蛋白在着床前胚胎中的表达强度进行定量分析。结果表明:BDNF与TrkB蛋白在发情周期及妊娠3.5天和4天子宫腔上皮、子宫腺上皮和血管内皮细胞中表达,在发情周期子宫内膜固有层基质细胞中不表达,但在妊娠3.5天和4天子宫蜕膜细胞中表达,发情前期、发情期和发情后期BDNF蛋白的表达强度高于发情间期;BDNF与TrkB蛋白在妊娠2天输卵管黏膜上皮和血管内皮细胞中表达;两种蛋白在着床前胚胎各发育阶段都有表达,在囊胚期达到最高水平。说明BDNF和TrkB蛋白通过旁分泌或自分泌途径对子宫、输卵管尤其是着床前胚胎发育发挥调控作用。
译  名：
The expression pattern of BDNF and TrkB in mouse uteri and the preimplantation embryos
作  者：
LIU Ben;College of Life Science and Resources Environment,Yichun University;
关键词：
mouse;;BDNF;;TrkB;;uterus;;preimplantation embryo;;immunohistochemistry
摘  要：
The aim of the present study was to observe the expression of BDNF and TrkB in mouse uteri and oviducts in the estrous cycle and early pregnancy as well as the preimplantation embryos. The expression of BDNF and TrkB were detected by immunohistochemistry and immunofluorescence staining,the expression level of BDNF and TrkB proteins in mouse preimplantation embryos were detected by image processing software. The results showed that the expression of BDNF and TrkB were found in mouse endometrial epithelial,glandular epithelium and blood vessel endothelium in the estrous cycle and days 3. 5 and 4 of pregnancy. There were not expressed in stromal cells in the estrus cycle,but expressed in decidual cells in days 3. 5 and 4 of pregnancy. The expression level of BDNF protein in the diestrum was lower than that in proestrus,estrus and metestrus. The expression of BDNF and TrkB protein was also existed in the tubal mucosa epithelial and endothelial cells in days 2 of pregnancy. Both proteins were expressed at each developmental stage of preimplantation embryos and reached their highest level in blastocyst stage. The results indicated that BDNF/TrkB signals played an important regulatory role in mouse uterus,fallopian tubes,especially preimplantation embryo development via paracrine or autocrine.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46