中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 野生毛葡萄水通道蛋白基因VhPIPs的克隆与组织特异性表达研究 园艺学报 2016,43 (12) 2304-2314
Position: Home > Articles > Cloning and Tissue Specificity Expression of the Aquaporin Genes from Wild Vitis quinquangularis Acta Horticulturae Sinica 2016,43 (12) 2304-2314

野生毛葡萄水通道蛋白基因VhPIPs的克隆与组织特异性表达研究

作  者：
李菲;颜培玲;张文娥;潘学军
单  位：
贵州大学贵州省果树工程技术研究中心
关键词：
毛葡萄;野生;水通道蛋白;基因克隆;表达
摘  要：
从野生毛葡萄组织中克隆了9个质膜水通道蛋白基因(VhPIP)的c DNA全长序列,并研究了其生物信息学特征及组织特异性表达。氨基酸序列分析表明,9个VhPIP分为PIP1和PIP2两类;均含有MIP蛋白家族保守的信号序列SGGHINPAVT和两个NPA(Asn-Pro-Ala)单元,均为稳定性蛋白、疏水性蛋白,且每个VhPIP均含有6个跨膜区。实时荧光定量RT-PCR分析表明,这9个VhPIP在抗旱的毛葡萄‘花溪–9’根、茎、叶中均有表达,其中VhPIP1;1、VhPIP1;2、VhPIP1;4、VhPIP2;1、VhPIP2;2和VhPIP2;4在其根中的表达量最多,可能与根部水分运输的调节密切相关;VhPIP1;5和VhPIP2;3在其叶中的表达量最多,可能主要参与叶片的水分运输,与叶片的蒸腾作用密切相关;VhPIP1;3在根、茎和叶中的表达量无显著性差异;相对于其他基因的表达,VhPIP1;1和VhPIP2;4在根中表达量最高,这两个基因可能在水分运输过程中起较大作用。
译  名：
Cloning and Tissue Specificity Expression of the Aquaporin Genes from Wild Vitis quinquangularis
作  者：
LI Fei;YAN Pei-ling;ZHANG Wen-e;PAN Xue-jun;Guizhou University,Guizhou Engineering Research Center for Fruit Crops;
关键词：
Vitis quinquangularis;;wild;;aquaporin;;gene cloning;;expression
摘  要：
Nine plasma membrane aquaporin genes named VhPIPs were cloned from wild Vitis quinquangularis and their bioinformatics features and tissue specificity expressions were studied in order to study the structural features of aquaporin gene in wild grape and its role in the process of drought resistance. Amino acid sequence analysis showed that these 9 VhPIP genes can be divided into 2 groups,PIP1 and PIP2,signal sequence SGGHINPAVT contain MIP protein family conserved and 2 NPA(Asn-Pro-Ala)units,every protein is stable and hydrophobic,and each VhPIP contains 6 trans membrane domains. The real-time RT-PCR result showed they expressed in roots,stems and leaves. And VhPIP1;1,VhPIP1;2,VhPIP1;4,VhPIP2;1,VhPIP2;2 and VhPIP2;4 had higher expression level in the roots of ‘Huaxi-9'than other tissues,they may be closely related to the regulation of root water transportation. VhPIP1;5 and VhPIP2;3 had the highest expression level in leaves,these 2 genes may be mainly related to the water transportation and the transpiration of leaf. The expression level of VhPIP1;3 had no significance in roots,stems and leaves. Compared to the expression of other genes,VhPIP1;1 and VhPIP2;4 had the highest expression in roots,which indicated that they may play a greater role in the water transportation.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46