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Position: Home > Articles > Prokaryotic expression of hfqgene fromListeria monocytogenes and polymers analysis of expressed Hfq protein Chinese Veterinary Science 2014 (2) 171-175

产单核细胞李氏杆菌hfq基因的原核表达及表达产物的聚合体分析

作  者:
殷月兰;付红;孔苏伟;谈卫军;赵丹;潘志明;陈祥;焦新安
单  位:
扬州大学
关键词:
产单核细胞李氏杆菌;Hfq蛋白;结构预测;多聚体
摘  要:
为了研究产单核细胞李氏杆菌Hfq蛋白的结构,将菌株yzuLM4的hfq基因在大肠杆菌中进行了表达。结果显示,在IPTG诱导下hfq基因获得可溶性表达,融合蛋白的大小约为16ku,诱导温度对表达的影响不显著。Western-blot分析结果表明,原核表达的Hfq蛋白具有反应原性。同时,通过非变性凝胶电泳,发现Hfq蛋白能以单体、二聚体、四聚体以及六聚体的形式存在,且各多聚体的比例不同。具有天然蛋白结构特征和免疫原性的Hfq蛋白的原核表达,为其在李氏杆菌中的功能研究奠定了基础。
译  名:
Prokaryotic expression of hfqgene fromListeria monocytogenes and polymers analysis of expressed Hfq protein
作  者:
YIN Yue-lan;FU Hong;KONG Su-wei;TAN Wei-jun;ZHAO Dan;PAN Zhi-ming;CHEN Xiang;JIAO Xin-an;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses/Jiangsu Key Laboratory of Zoonosis/Yangzhou University;
关键词:
Listeria monocytogenes;;Hfq protein;;structure prediction;;polymer
摘  要:
To structurally analyze Hfq of Listeria monocytogenes,hfq gene from L.monocytogenes strain yzuLM4was expressed in Escherichia coli.The predicted 16ku soluble fusion protein His-Hfq was detected by SDS-PAGE after IPTG induction of recombinant bacteria,and the level of expression was not affected significantly by induction temperatures.Western-blot analysis showed that His-Hfq had reactionogenicity.There were 3polymeric forms of the His-Hfq protein,including dimer,tetramer and hexamer as shown by Native-PAGE.This study successfully expressed and purified Hfq protein with immunogenicity in E.coli,which could be used to explore the relationship between the structure and function of Hfq in L.monocytogenes.

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