作 者：
闫晓刚;张芳毓;郎洪彦;金香淑;赵中利;刘臣
关键词：
人参茎叶多糖;蛋雏鸡;小肠黏膜发育;免疫细胞
摘 要：
为研究人参茎叶多糖(GSLP)对雏鸡小肠黏膜组织发育和免疫细胞的影响,选择600只体重相近(52.79±4.58g)的7日龄健康海兰褐雏鸡,随机分为10个处理,每个处理60只(3个重复,每个重复20只),分为正常饲喂组和免疫抑制组,各包括5个处理,GSLP添加量分别为0、0.2、0.4、0.8和1.6g·kg-1,试验期35d。饲养期结束后,每个处理中随机选择9只雏鸡(每个重复3只),采集空肠和回肠样品。结果显示:(1)GSLP提高了空肠绒毛宽度和表面积(P<0.01),0.8g·kg-1 GSLP降低空肠绒毛高度和隐窝深度(P<0.05),0.8和1.6g·kg-1 GSLP降低回肠绒毛高度和隐窝深度(P<0.01),GSLP对绒腺比无显著影响;(2)空肠绒毛高度(P<0.05)和黏膜厚度(P<0.01)以及回肠绒毛高度(P<0.05)中存在GSLP与免疫状态之间的交互作用;(3)0.4、0.8和1.6g·kg-1 GSLP增加空肠CD3+阳性细胞的面积(P<0.05),1.6g·kg-1 GSLP提高空肠肥大细胞的数量(P<0.05),GSLP对免疫细胞的作用存在剂量效应。结果表明,GSLP对小肠黏膜发育具有一定的促进作用,但对养分吸收机能并无显著影响;0.4~1.6g·kg-1 GSLP提高空肠的黏膜免疫水平;GSLP对环磷酰胺前期抑制的空肠绒毛高度和黏膜厚度的发育具有改善作用。
译 名：
Effects of Ginseng Stem and Leaf Polysaccharide on Small Intestinal Mucous Tissue Development and Immune Cells of Chicken
作 者：
YAN Xiao-gang;ZHANG Fang-yu;LANG Hong-yan;JIN Xiang-shu;ZHAO Zhong-li;LIU Chen;The Branch Academy of Animal Science,Jilin Academy of Agricultural Science;
关键词：
ginseng stem and leaf polysaccharide;;egg laying chicken;;small intestinal mucous development;;immune cells
摘 要：
To study the effects of ginseng stem and leaf polysaccharide(GSLP)on small intestinal mucous tissue development and immune cells of chicken,600 of 7days healthy Hy-line Variety Brown chicken(52.79±4.58g)were freely divided into 10 treatments,60chicken per treatment(3replications,20 chicken per replication),5treatments were included in normal fed group and others were in immune depressed group.The additive levels of GSLP were 0,0.2,0.4,0.8and1.6g·kg-1 in each group based on basal diet.After feeding stage,9chicken of each treatment(3per replication)were randomly selected for samples collection of jejunum and ileum.The results showed that:(1)GSLP improved villus width and surface area of jejunum(P<0.01),0.8and 1.6g·kg-1 GSLP decreased villus height and recess depth of ileum(P<0.01),but GSLP had no significant effects on villus to gland ratio;(2)The interactions of GSLP with immune state were observed in jejunum villus height(P<0.05)and mucous thickness(P<0.01),and ileumvillus height(P<0.05);(3)0.4,0.8and 1.6g·kg-1 GSLP increased the amounts of CD3+T lymphocyte(P<0.05),and 1.6g·kg-1 GSLP increased the amounts of mast cell(P<0.05)in jejunum,and dosage effects were existed in the influence of GSLP on immune cells.In conclusion,GSLP had somewhat improvement on small intestinal mucous tissue development,but had no significant influence on nutrients absorptive function;0.4to 1.6g·kg-1 GSLP increased the level of jejunum mucous immunization;and GSLP could improve the development of jejunum villus height and mucous thickness depressed by cyclophosphamide in early trial stage.
