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当前位置: 首页 > 文章 > 氟对大鼠支持细胞紧密连接occludin基因与蛋白的影响 山西农业科学 2017,45 (4) 518-521
Position: Home > Articles > Effect of Fluorine on Sertoli Cell Tight Junction occludin Gene and Protein in Rat Journal of Shanxi Agricultural Sciences 2017,45 (4) 518-521

氟对大鼠支持细胞紧密连接occludin基因与蛋白的影响

作  者：
张晓燕;张慧慧;程敏;孙子龙;王俊东
单  位：
山西农业大学动物科技学院
关键词：
氟化钠;支持细胞;紧密连接;基因;蛋白
摘  要：
试验旨在研究NaF对支持细胞occudin基因和蛋白表达的影响。采用不同浓度的NaF(0,0.1,1,10 mg/L)分别处理大鼠睾丸支持细胞48 h,q RT-PCR检测occludin基因水平的变化,免疫荧光技术检测occludin蛋白定位及水平的变化。结果显示,与对照相比,10 mg/L NaF染毒,occludin mRNA相对表达量显著下降(P<0.05);0.1,1.0 mg/L NaF染毒,occludin mRNA相对表达量下降,但差异不显著;occludin蛋白随着染氟剂量的增加,表达量逐渐降低,且occludin蛋白由细胞膜转移到细胞核附近的部位。说明NaF对支持细胞紧密连接的破坏可能与occudin基因与蛋白表达的变化有关。
译  名：
Effect of Fluorine on Sertoli Cell Tight Junction occludin Gene and Protein in Rat
作  者：
ZHANG Xiaoyan;ZHANG Huihui;CHENG Min;SUN Zilong;WANG Jundong;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;
关键词：
sodium fluoride;;sertoli cell;;tight junction;;gene;;protein
摘  要：
The aim of this study was to figure out the effect of NaF on sertoli cell occludin gene and protein expression. Different concentrations of NaF(0, 0.1, 1.0, 10 mg/L)were used to treat rat testis sertoli cells for 48 h, then detected occludin gene changes by q RT-PCR, the protein changes by immunofluorescence technique. The result showed that the relatire expression of occludin m RNA decreased remarkbly(P<0.05), when the cell was treated with 10 mg/L NaF, compared with other groups. When the cell was treated with0.1, 1.0 mg/L NaF, the relative expression of occludin m RNA decreased, but the difference was not significant. And occludin protein expression gradually decreased and transferred from cell membrane to near the nucleus with the increasing dose of NaF. It illustrates that broken cell tight junction caused by NaF may be associated with the changes of occludin gene and protein expression.

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