当前位置: 首页 > 文章 > α-萘酚-硫酸法测定酒糟多糖含量 食品科学 2013,34 (18) 245-248
Position: Home > Articles > Determination of Polysaccharides from Vinasse by α-Naphthol-Sulfuric Acid Method FOOD SCIENCE 2013,34 (18) 245-248

α-萘酚-硫酸法测定酒糟多糖含量

作  者:
张世仙;刘焱;朱彬;金茜;曾启华
单  位:
遵义师范学院化学化工学院,黔北特色资源应用研究实验室;遵义师范学院化学化工学院
关键词:
酒糟;多糖;α-萘酚-硫酸法
摘  要:
目的:从酒糟中提取多糖,并建立一种操作简单、结果可靠的检测酒糟多糖含量的方法。方法:采用酶法提取酒糟多糖,用α-萘酚-硫酸显色法测定多糖含量。结果:溶液最大吸收波长为560nm,在6~36μg/mL范围内吸光度与被测物含量呈良好的线性关系,R2=0.9975,平均加标回收率100.65%,以葡萄糖计,酒糟中多糖含量为10.6%,RSD为4.0%。结论:α-萘酚-硫酸显色法测定酒糟多糖含量步骤简单,重复性好,结果可靠。
译  名:
Determination of Polysaccharides from Vinasse by α-Naphthol-Sulfuric Acid Method
作  者:
ZHANG Shi-xian;LIU Yan;ZHU Bin;JIN Qian;ZENG Qi-hua;Laboratory of Utilization Research on Characteristic Resources in Qianbei,School of Chemistry and Chemical Engineering,Zunyi Normal College;
关键词:
vinasse;;polysaccharides;;α-naphthol-sulfuric acid method
摘  要:
Objective: To extract polysaccharides from vinasse(byproduct of Chinese liquor making) and to establish a simple and reliable determination method.Methods: Vinasse polysaccharides were extracted by an enzymatic method,and α-naphthol-sulfuric acid method was used for the determination of polysaccharide content.Results: The maximum absorption wavelength was 560 nm.There was a good linear relationship between absorbance and glucose concentration in the range from 6 to 36 μg/mL(R2= 0.9975).The average spiked recovery rate was 100.65%.The content of polysaccharides from vinasse(caculated as glucose) was 10.6%(RSD = 4.0%).Conclusion: The α-naphthol-sulfuric acid method is a simple and reliable assay with good reproducibility for determining polysaccharides from vinasse.

相似文章

计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 1

所属期刊

推荐期刊