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Position: Home > Articles > Screening and Inhibitory Activity of Endophytic Fungi from Acorus calamus L. FOOD SCIENCE 2010,31 (9) 211-215

水菖蒲内生菌分离与抗菌活性菌株筛选

作  者:
周晓坤;陈钧;韩邦兴;何佳;刘长风
单  位:
江苏大学药学院
关键词:
水菖蒲;内生菌;抗菌活性
摘  要:
对水菖蒲内生真菌进行分离,并进行抗菌活性的筛选。采用改良氯化三苯四氮唑显色(TTC)微量稀释法筛选抗菌活性菌株。以18种常见导致食品腐败的细菌、酵母菌和霉菌为指示菌研究活性菌株的抗菌能力。结果显示,水菖蒲内存在广泛的具有抗菌活性的内生菌,占内生菌总数的40.7%,高活性菌株占内生菌总数的12.39%。其中S9菌株发酵液具有抗细菌、酵母菌和丝状真菌的广谱抗菌能力,是潜在的食品防腐抗菌资源。
译  名:
Screening and Inhibitory Activity of Endophytic Fungi from Acorus calamus L.
作  者:
ZHOU Xiao-kun1,CHEN Jun1,*,HAN Bang-xing1,2,HE Jia1,LIU Chang-feng1(1.College of Pharmacy,Jiangsu University,Zhenjiang 212013,China ;2.Anhui Engineering Technology Research Center of Plant Cell Engineering,Lu'an 237012,China)
关键词:
Acorus calamus L.;endophytic fungi;antibacterial activity
摘  要:
In order to isolate and screen endophytic fungi with inhibitory activity from Acorus calamus L.,active strains were screened through modified TTC micro-dilution method.MIC(minimum inhibitory concentration) and MBC(minimum bactericidal concentration) were detected using 18 food spoilage strains.Results indicated that 40.7% endophytes exhibited antimicrobial activity and 12.39% endophytes exhibited higher antimicrobial activity.In addition,strain S9 exhibited the broadspectrum antimicrobial activity to bacteria,yeast and mycelia fungi.Active endophytic strains from A.calamus L.are potential resources for food preservation.

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