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Position: Home > Articles > Influence of Spray-drying on Human Lysozyme Transgenic Goat Milk Journal of Sichuan Agricultural University 2013,31 (2) 204-207

喷雾干燥对人溶菌酶转基因山羊乳汁的影响

作  者:
吴敏;刘思国;兰翀;徐旭俊;陈建泉;马恒东;成国祥
单  位:
上海转基因动物育种与制药工程技术研究中心/上海杰隆生物工程股份有限公司;四川农业大学动物科技学院
关键词:
喷雾干燥;人溶菌酶;转基因山羊
摘  要:
【目的】揭示奶粉制备过程对转基因山羊乳汁中表达的重组人溶菌酶(recombinant human lysozyme,rhLYZ)的影响。【方法】对表达有2mg/mL rhLYZ的转基因山羊乳汁进行了喷雾干燥,对获得的奶粉样品进行了溶解性,乳汁成分,蛋白组分以及溶菌酶活性的测定,对测定结果进行统计分析。【结果】喷雾干燥引发非脂固形物的损失率为42.05%;整个热处理过程引发可溶性蛋白损失率为25.3%;乳汁中rhLYZ的损失为51.1%,扣除总干物质的损耗,喷雾干燥过程引发了约17.7%的rhLYZ生物学活性损失。【结论】制备奶粉过程对转基因山羊乳汁中表达的人溶菌酶影响较小,喷雾干燥是人溶菌酶羊奶的一种可行的保存和加工方式。
译  名:
Influence of Spray-drying on Human Lysozyme Transgenic Goat Milk
作  者:
WU Min1,LIU Si-guo2,LAN Chong2,XU Xu-jun2, CHEN Jian-quan2,MA Heng-dong1,CHENG Guo-xiang2(1.College of Animal Science and Technology,Sichuan Agricultural University,Yaan 625014,Sichuan,China; 2.Shanghai Engineering Research Center for Transgenic Animal breeding and Pharmaceutical/Shanghai Genon Bioengineering Co.Ltd.,Shanghai 201203,China)
关键词:
spray-drying;human lysozyme;transgenic goat
摘  要:
【Objective】 The influence of milk powder preparation process on the physicochemical properties of transgenic goat milk and the recombinant human lysozyme(rhLYZ) were studied.【Method】 The transgenic goat milk with 2 mg/mL of rhLYZ was treated by spray-drying.The solubility,milk composition,protein fractions,and lysozyme activity of milk samples were tested.The results were statistical analyzed.【Results】 The lose rate of non-fat solids caused by spray-drying was up to 42.05%;the lose rate of dissolvable proteins was about 25.3%;and about 17.7% of rhLYZ biological activity was losted which resulted from the drying process.【Conclusion】 The influence of milk powder preparation process on rhLYZ was mild,and the milk powder could be a feasible method for preserving and processing rhLYZ milk.

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