Position: Home > Articles > A Biosensor Based on Immobilizing Hemoglobin on Quaternized Poly(4-vinyl Pyridine) and Electrodeposited Pt-Nanoparticles as Matrixes
Journal of Southwest University(Natural Science Edition)
2010,32
(3)
39-44
基于季铵化聚乙烯吡啶和纳米铂固定血红蛋白的过氧化氢生物传感器的研究
作 者：
滕瑛丽;刘昌华;郭晓利
单 位：
西南大学化学化工学院
关键词：
纳米铂;电沉积;季铵化聚乙烯吡啶;血红蛋白
摘 要：
将血红蛋白和季铵化聚乙烯吡啶按一定比例混合,滴涂在表面修饰了纳米铂的玻碳电极上,成功制备了性能良好的过氧化氢传感器.通过采用循环伏安法(CV)和计时电流法考察了该传感器的电化学性质.最佳实验条件下,其峰电流值与H2O2浓度在3.5×10-6~3.15×10-3mol/L成良好的线性关系,检测下限为1.5×10-6mol/L(S/N=3),相关系数为0.999 2.实验证明该电极对过氧化氢具有响应快,灵敏度高、线性范围宽和稳定性好的性能.
译 名：
A Biosensor Based on Immobilizing Hemoglobin on Quaternized Poly(4-vinyl Pyridine) and Electrodeposited Pt-Nanoparticles as Matrixes
作 者：
TENG Ying-li,LIU Chang-hua,GUO Xiao-li School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China
关键词：
platinum nanoparticle;electrodeposition;quaternized poly(4-vinyl pyridine)(QPVP);hemoglobin(Hb)
摘 要：
A highly efficient H2O2 biosensor was fabricated based on the mixture of hemoglobin(Hb) and quaternized poly(4-vinyl pyridine)(QPVP) and nano-Pt modified glassy carbon electrode.The electrochemical characteristics of the biosensor were studied by cyclic voltammetry(CV) and chronoamperometry.Under optimized conditions,the biosensor exhibited a dynamic range of 3.5×10-6 to 3.15×10-3 mol/L to H2O2 with a detection limit of 1.5×10-6 mol/L and a correlation coefficient of 0.999 2.Moreover,the biosensor exhibited high sensitivity to H2O2,good repeatability,selectivity and stability.
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