Position: Home > Articles > Double Membrane Amplification of the Amperometric Immunosensor for Sensing Carcinoembryonic Antigen
Journal of Shihezi University(Natural Science)
2012,30
(4)
118-122
双层膜放大的电流型癌胚抗原免疫传感器的研究
作 者：
王丽;孙涛;谭超
单 位：
宜宾学院化学与化工学院
关键词：
纳米铂;普鲁士蓝;癌胚抗原;免疫传感器
摘 要：
以金电极为电极材料,纳米铂/普鲁士蓝复合物膜为固载基底,癌胚抗体(anti-CEA)为模板免疫蛋白质分子,研制成一种高灵敏电流型癌胚抗原免疫传感器。该传感器以辣根过氧化氢酶(HRP)代替牛血清白蛋白封闭非特异性结合位点,放大了电流信号,提高了电极的灵敏度,并采用循环伏安技术对电极的制备过程进行了研究。在优化的实验条件下,该传感器响应的峰电流值与癌胚抗原浓度在1.0～80.0ng/mL的范围内保持良好的线性关系,检测限为0.4ng/mL。
译 名：
Double Membrane Amplification of the Amperometric Immunosensor for Sensing Carcinoembryonic Antigen
作 者：
WANG Li,SUN Tao,TAN Chao(College of Chemistry and Chemical Engineering,Yibin University,Yibin 644007,China)
关键词：
platinum nanoparticle;prussian blue;carcinoembryonic antigen;immunosensor
摘 要：
A new amperometric immunosensor for the determination of carcinoembryonic antigen(CEA) was developed by means of immobilizing carcinoembryonic antibody onto the nano-Pt/ Prussian-blue-modified composite film.The research substituted Horseradish peroxidase(HRP) for bovine serum albumin(BSA) to block nonspecific binding sites and amplify the current signal of the antigen-antibody reaction.Cyclic voltammetry(CV) was employed to characterize the assembly process.Under the optimized conditions,the response current of the proposed immunosensor has a good linear relationship with CEA in the concentration ranges from 1.0 to 80.0 ng/mL with a detection limit of 0.2 ng/mL.
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