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杀真菌素链霉菌变温发酵生产恩拉霉素的研究

作  者:
潘春梅;王静;张晓静
单  位:
河南牧业经济学院生物工程系
关键词:
恩拉霉素;杀真菌素链霉菌;变温发酵;正交试验设计
摘  要:
试验以杀真菌素链霉菌为生产菌株,系统研究了不同温度对菌体生长、总糖消耗和恩拉霉素生物合成的影响,在此基础上提出了分阶段变温控制策略,并利用正交试验设计法对变温发酵的主要工艺参数进行了优化。试验发现后期培养温度对菌株发酵生产恩拉霉素的影响最为显著,优化后的变温发酵控制条件为:发酵初期温度控制在31℃培养,70 h后改在27℃培养至发酵结束。采用优化后的变温培养工艺,恩拉霉素效价达到6 687 U/ml,与恒温29℃培养条件相比较,恩拉霉素产量提高了13%。
译  名:
Study on variable temperature control strategy of enramycin production by streptomyces fungicidicus
作  者:
Pan Chunmei;Wang Jing;Zhang Xiaojing;
关键词:
enramycin;;streptomyces fungicidicus;;temperature-shifting fermentation;;orthogonal experimental design
摘  要:
In this paper,the effects of temperature on the cell growth,sugar consumption and enramy-cin production with streptomyces fungicidicus L12 were investigated.Based on the results,a tempera-ture-shifted controlling mode for enramycin production was presented and the optimum fermentationcondition was established by orthogonal experimental design.The experiment results indicate that en-ramycin fermentation was remarkable influenced by the temperature of later stage,and the optimal tem-perature-shifted strategy is that culture is performed at 31 ℃ for 70 h,then the temperature was shifted to27 ℃,and kept up till the end of the fermentation process.By this method,the maximum enramycinyield reached 6 687 U/ml,which was high 13% than under constant temperature(29 ℃) fermentation mode.

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