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Position: Home > Articles > Study on Extraction and Purification Technology of Chlorogenic Acid from Lonicera japonica Thunb Journal of Anhui Agricultural Sciences 2010,31 (24) 132-133+137

分离纯化金银花绿原酸工艺研究

作者：

Fan Yuan-jing;Xiaowei Xu;Yu FangFang;Huimin Gao;Ping Zhang;Fang TongHui;Xiaowei H

单位：

Huangshan Yaogu Science and Technology C;Huangshan Yaogu Science and Technology Co

关键词：

chlorogenic acid;extraction and purification technology;optimum;eluent;ph;6

摘要：

[目的]研究从金银花中提取、纯化绿原酸的工艺。[方法]通过单因素试验和正交试验,筛选最佳提取条件和纯化条件。[结果]最佳提取工艺条件为:提取温度60℃,提取溶剂为60%乙醇(V/V),pH值为4,提取时间为1.5h,料液比为1∶25。在此条件下,提取的绿原酸得率为4.52%。最佳纯化工艺条件为:洗脱液浓度20%,洗脱液体积为5BV,洗脱液pH值为8,洗脱液流速为5ml/min。在此条件下,纯化的绿原酸产品纯度为58.3%。[结论]该试验得到了绿原酸最佳提取条件和纯化条件,为绿原酸的工业化生产提供了理论试验依据。

译名：

Study on Extraction and Purification Technology of Chlorogenic Acid from Lonicera japonica Thunb

作者：

FAN Yuan-jing et al (College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei,Anhui 230009)

关键词：

Lonicera japonica Thunb.;chlorogenic acid;macroporous resin;Extraction;Purification

摘要：

[Objective]The aim was to study the extraction and purification technology for Lonicera japonica Thunb.. [Method]The optimum extraction and purification technology were obtained from the single factor test and the orthogonal test respectively. [Result]The optimum extraction conditions were as follows:extraction temperature of 60 ℃,pH of 6,ethanol concentration of 60%,material-liquid ratio of 1:25 and extraction time of 1.5 h,under which the yield of chlorogenic acid was 4.6%. The optimum purification conditions were as follows:eluent concentration of 20%,eluent volume of 5 BV,eluent pH of 8 and flow rate of 5 ml/min,under which the purity of chlorogenic acid was 58.3%. [Conclusion]The optimum extraction and purification technology were obtained,which will provide experimental evidence for the industrialized production of chlorogenic acid.

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分离纯化金银花绿原酸工艺研究

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