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当前位置: 首页 > 文章 > Enterobacter sp.对土壤莠去津污染修复及代谢途径分析 农药 2017 (12) 887-889
Position: Home > Articles > Effect of Enterobacter sp. on Soil Atrazine Remediation and Metabolic Pathway Analysis Agrochemicals 2017 (12) 887-889

Enterobacter sp.对土壤莠去津污染修复及代谢途径分析

作  者：
刘丹丹;刘畅;王琳;赵晋;丁永昌
单  位：
沈阳化工大学环境与安全工程学院
关键词：
莠去津;土壤污染;生物修复;微生物
摘  要：
[目的]明确莠去津降解菌对土壤污染的修复效果,并分析代谢过程。[方法]通过土壤修复试验,筛选莠去津污染修复的最优环境条件。利用高通量基因测序方法,分析莠去津的代谢过程。[结果]在接菌量为2%(v/v,OD600为1)、温度为25℃、时间为28 d时,修复效果最好。菌株对莠去津的降解是在6种酶及其辅助蛋白的共同作用下完成,生成5个中间产物。[结论]试验菌株具有较高的莠去津污染修复能力和独特的代谢过程。
译  名：
Effect of Enterobacter sp. on Soil Atrazine Remediation and Metabolic Pathway Analysis
作  者：
LIU Dan-dan;LIU Chang;WANG Lin;ZHAO Jin;DING Yong-chang;Department of Environmental and Safety Engineering, Shenyang University of Chemical Technology;
单  位：
Department of Environmental and Safety Engineering, Shenyang University of Chemical Technology
关键词：
atrazine;;soil contamination;;bioremediation;;microorganism
摘  要：
[Aims] The aims were to clarify the effects of atrazine degrading bacteria on soil pollution and analyze its metabolic process. [Methods] The optimum environmental conditions for atrazine removal were screened by soil remediation test. The high-throughput gene sequencing method was used to analyze the metabolic process of atrazine degradation. [Results] The results showed that the remediation effect was the best when the amount of bacteria was 2%(v/v, OD_(600)=1), the temperature was 25 ℃ and the time was 28 days. Atrazine degradation by the strain was carried out under the combined action of 6 enzymes and their auxiliary proteins, and 5 intermediate products formed.[Conclusions] The test strain has high ability of contaminant remediation and unique metabolic process.

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