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当前位置: 首页 > 文章 > 甜荞·苦荞和大野荞的高分子量种子蛋白亚基研究 安徽农业科学 2010,38 (33) 18678-18680
Position: Home > Articles > Study on HMW Seed Protein Subunits in Common Buckwheat,Tartary Buckwheat and Fagopyrum megaspartanium Accessions Journal of Anhui Agricultural Sciences 2010,38 (33) 18678-18680

甜荞·苦荞和大野荞的高分子量种子蛋白亚基研究

作  者：
王燕;陈庆富
单  位：
贵州师范大学生命科学学院植物遗传育种研究所
关键词：
甜荞;苦荞;大野荞;SDS-PAGE;种子蛋白亚基
摘  要：
[目的]探讨甜荞、苦荞和大野荞的高分子量种子蛋白亚基。[方法]利用SDS-PAGE电泳法对18个不同地方居群的大野荞、3个甜荞和3个苦荞收集系的高分子量种子蛋白亚基进行研究。[结果]从这3个物种的24个收集系中,在高分子量区域(20~70 kDa)共发现12个不同的种子蛋白亚基谱带,种间差异大,种内差异小。3个物种在种子蛋白亚基分布上存在一定的相似性,约30%的谱带相同。其中,甜荞发现有9个谱带,含1个独特谱带(SSP33.71);苦荞有8个谱带,含不同于甜荞的2个独特谱带(SSP39.15和SSP29.35);大野荞有7个谱带,不仅含有苦荞的独特谱带,而且其谱带分布与苦荞极为相似。[结论]该研究为荞麦品质遗传育种、种间系统关系、高品质种子蛋白亚基基因工程等研究提供指导。
译  名：
Study on HMW Seed Protein Subunits in Common Buckwheat,Tartary Buckwheat and Fagopyrum megaspartanium Accessions
作  者：
WANG Yan et al (Institute of Plant Genetics and Breeding,School of Life Science,Guizhou Normal University,Guiyang,Guizhou 550001)
关键词：
Common buckwheat;Tartary buckwheat;Fagopyrum megaspartanium;SDS-PAGE;Seed protein subunit
摘  要：
[Objective] The study aimed to discuss the HMW seed protein subunits in common buckwheat,tartary buckwheat and Fagopyrum megaspartanium accessions.[Method] The HMW seed protein subunits of 18 wild buckwheat accessions of F.megaspartanium native to in different places and 6 common buckwheat and tartary buckwheat accessions were studied by means of SDS-PAGE.[Result] There were totally 12 seed protein subunit bands in high molecular weight region(20-70 kDa)discovered in 24 accessions from 3 species,with great variation among different species and little variation within the same species.3 species had similar spectrum of seed protein subunits and about 30% bands were same.Among them,The common buckwheat had 9 bands including a special band(SSP33.71) different from others.Tartary buckwheat had 8 bands including 2 special bands(SSP39.15 and SSP29.35) different from common buckwheat.F.megaspartanium had 7 bands,which not only include those special bands of tartary buckwheat,but also its band distribution was similar with that of tartary buckwheat.[Conclusion] The study provided the guidance for the studies on quality genetic breeding,interspecies system relations,gene engineering of buckwheat seed protein subunits.

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