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Position: Home > Articles > Establishment of RT-PCR and Real-time RT-PCR Detection Method for Simian Retrovirus Progress in Veterinary Medicine 2013 (12) 57-60

猴逆转录病毒RT-PCR和Real-time RT-PCR检测方法的建立

作  者:
熊炜;蒋静;张强;盘宝进;李健;魏晓锋;黄忠荣;胡建华
单  位:
上海出入境检验检疫局;广西出入境检验检疫局;上海实验动物研究中心
关键词:
猴;猴逆转录病毒;RT-PCR;real-time RT-PCR;Taq Man探针
摘  要:
猴逆转录病毒(Simian type D retrovirus,SRV)是引起猴获得性免疫缺陷综合征(Simian acquired immunodeficiency syndrome,SAIDS)的病原之一,其严重危害猴的健康,并威胁与猴接触人员的健康,是无特定病原体(SPF)猴必须排除的病毒之一。为了应对口岸对进出境野生及实验用灵长类动物SRV感染情况的监测和流行病学调查的需要,建立了RT-PCR和real-time RT-PCR检测SRV的方法,并对方法的特异性、敏感性和稳定性进行了验证。
译  名:
Establishment of RT-PCR and Real-time RT-PCR Detection Method for Simian Retrovirus
作  者:
XIONG Wei;JIANG Jing;ZHANG Qiang;PAN Bao-jin;LI Jian;WEI Xiao-feng;HUANG Zhong-rong;HU Jian-hua;Shanghai Entry-Exit Inspection and Quarantine Bureau;Guangxi Entry-Exit Inspection and Quarantine Bureau;Shanghai Laboratory Animal Research Center;
关键词:
monkey;;Simian type D retrovirus;;RT-PCR;;real-time RT-PCR;;Taq Man probe
摘  要:
Simian type D retrovirus(SRV)is one of pathogens led to simian acquired immunodeficiency syndrome(SAIDS),which is harmful for health of monkey and its engaged person.And it is also one of virus must be eliminated from specefic pathogen free(SPF)monkey.In order to survey and investigate the epidemic character of SRV transmission among wild and experimental monkeys,methods of RT-PCR and real-time RT-PCR using Taq Man probe to detect SRV were established and proved to be specific with high sensitivity and stability.

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