作 者：
王东升;张世栋;董书伟;苗小楼;魏立琴;邝晓娇;那立冬;闫宝琪;严作廷
单 位：
中国农业科学院兰州畜牧与兽药研究所/农业部兽用药物创制重点实验室/甘肃省中兽药工程技术研究中心/甘肃省新兽药工程重点实验室
关键词：
藿芪灌注液;急性毒性;长期毒性;最大给药量;小鼠;大鼠
摘 要：
为了测定小鼠口服藿芪灌注液的急性毒性和长期毒性,评价其安全性,为临床安全用药提供依据,采用最大给药量法进行了急性毒性研究,选择健康小鼠20只,24 h内按40 g/kg体重灌胃给药3次,给药后连续观察10天,测定其1日最大给药量,确定藿芪灌注液的急性毒性。将80只健康大鼠随机分为高、中、低剂量组和对照组,每组雌雄各10只,高、中、低剂量组分别按20、10、3 g/kg体重灌服藿芪灌注液,对照组按20 m L/kg灌服生理盐水,每天一次,连续给药35天,通过临床观察、病理组织学检查、血液生理生化指标测定,研究其长期毒性。结果表明,小鼠口服藿芪灌注液的1日最大给药量为120 g/kg,相当于临床奶牛日用量的600倍。藿芪灌注液不影响大鼠的采食、活动、饮水、增重、脏器指数、血液生理生化指标,不会引起大鼠发病和死亡。说明藿芪灌注液实际无毒,至少在20 g/kg体重给药剂量下大鼠连续给药35天是安全的。
译 名：
Toxicity of Huoqi Perfusate
作 者：
Wang Dongsheng;Zhang Shidong;Dong Shuwei;Miao Xiaolou;Wei Liqin;Kuang Xiaojiao;Na Lidong;Yan Baoqi;Yan Zuoting;Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS/Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture/Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province /Key Laboratory of New Animal Drug Project of Gansu Province;
关键词：
huoqi perfusate;;acute toxicity;;long-term toxicity;;maximum daily dose;;mouse;;rat
摘 要：
To evaluate safety of huoqi perfusate and provide evidence for clinical dosage, acute toxicity andlong-term toxicity of huoqi perfusate were determined by intragastric administration. Acute toxicity trial wastaken on 20 mice which were subjected to gastric perfusion with huoqi perfusate of 40 g per kilogram bodyweight 3 times in 24 hours according to the maximum daily dose. To determine the maximum daily dose, themice were observed for 10 days after the medicine was administered. Long-term toxicity trial was carried outon 80 rats which were randomly divided into three treatment groups and one control group with 10 females and10 males in each group. The rats in three treatment groups, which were high-dose group, middle-dose groupand low-dose group, were respectively administered huoqi perfusate by intragastric administration 20, 10 and3 g per kilogram body weight everyday for 35 consecutive days. Huoqi perfusate was replaced by 20 m L/kgnormal saline in the control group. To determine long- term toxicity, clinical observation, histopathologicalexamination and the measurement of blood physiology and biochemical indicators were conducted. The results showed that the maximum daily dose of intragastric administration of huoqi perfusate in mice was 120 g/kg,which was 600 times of the daily dose of dairy cows. Huoqi perfusate did not affect foraging, activity, drinking,weight gain, organ index and blood physiology and biochemical indicators of rats, and would not causemorbidity and mortality in rats, indicating that intragastric administering huoqi perfusate by 20 g per kilogrambody weight for 35 consecutive days was safe for rats.
