作 者：
乔宇;廖李;汪兰;吴文锦;陈学玲;王俊;丁安子;何建军;梅新;程薇
单 位：
湖北省农业科技创新中心农产品加工分中心/湖北省农业科学院农产品加工与核农技术研究所
关键词：
山药(Dioscorea opposita);山药皮;山药粉;尿囊素;液相色谱
摘 要：
以山药(Dioscorea opposita)皮和山药粉为原料,通过水超声波提取尿囊素,采用Agilent ZORBAX Ecilipse XDB-C18液相色谱柱,以0.025 mol/L磷酸二氢钠缓冲液(p H 3.0)-甲醇(95/5,V/V)为流动相,检测波长224 nm,建立了山药粉和山药皮中尿囊素的测定方法,结果表明该方法在0.1~1 mg/m L范围内与峰面积呈良好线性关系,平均回收率为96.77%,RSD为1.90%,该法条件稳定、操作简单、精密度及回收率较好,定量结果准确可靠,适用于分析3种山药的山药皮和山药粉中的尿囊素含量。
译 名：
Determination of Allantion in Different Chinese Yam Varieties
作 者：
QIAO Yu;LIAO Li;WANG Lan;WU Wen-jin;CHEN Xue-ling;WANG Jun;DING An-zi;HE Jian-jun;MEI Xin;CHENG Wei;Agricultural Products Processing Subcenter of Hubei Agricultural Science&Technology Innovation Center / Research Institute of Agricultural Products Processing and Nuclear-Agricultural Technology , Hubei Academy of Agricultural Sciences;
关键词：
Dioscorea opposite;;yam peel;;yam;;allantion;;HPLC
摘 要：
A quantitative HPLC method for determining allantion in the power and peel of Dioscorea opposita(Chinese Yam)samples was established. The yam power and yam peel were extracted with ultrasonic wave and separated by Agilent ZORBAX Ecilipse XDB-C18 column with the mobile phase of sodium acetate buffer(p H 3.0)-methanol(95∶5, V / V).The wavelength of detection was 224 nm. The results showed that the linearity range was between 0.1~1 mg / m L. The average recovery rate were 96.77%. The RSD was 1.90%. The established method is stable, simple, accurate with good recovery. It is useful for analyzing the allantion in three yam powers and yam peels.
