当前位置: 首页 > 文章 > 不同病原菌胁迫下青蛤血淋巴转录组中免疫相关基因的比较 安徽农业科学 2018,46 (27) 84-87
Position: Home > Articles > Comparison of Immune-related Genes in Transcriptome Library of Cyclina sinensis Hemolymph under the Stress of Different Pathogenic Bacteria Journal of Anhui Agricultural Sciences 2018,46 (27) 84-87

不同病原菌胁迫下青蛤血淋巴转录组中免疫相关基因的比较

作  者:
赵婷;潘宝平
单  位:
天津医学高等专科学校;天津师范大学生命科学学院
关键词:
青蛤;转录组文库;鳗弧菌;藤黄微球菌;免疫相关基因
摘  要:
利用病原微生物革兰氏阳性藤黄微球菌(Micrococcus luteus)和革兰氏阴性鳗弧菌(Vibrio anguillarum)分别侵染活体青蛤,采用第二代测序Mi Seq技术测序,构建了青蛤血淋巴中应答2种病原物的转录组文库。根据KEGG等数据库注释信息对Unigene进行生物学通路注释,预测出青蛤免疫信号通路的差异性表达基因及相关注释信息。结果发现,共注释并筛选出2 605个差异表达基因,其中893个基因注释到15个免疫相关的通路中。该研究可为探索青蛤的免疫识别方式和信号传导路径提供依据。
译  名:
Comparison of Immune-related Genes in Transcriptome Library of Cyclina sinensis Hemolymph under the Stress of Different Pathogenic Bacteria
作  者:
ZHAO Ting;PAN Bao-ping;Tianjin Medical College;Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Sciences,Tianjin Normal University;
关键词:
Cyclina sinensis;;Transcriptome library;;Vibrio anguillarum;;Micrococcus luteus;;Immune-related genes
摘  要:
Cyclina sinensis were injected with Micrococcus luteus and Vibrio anguillarum respectively. Using the Illumina Miseq system of second-generation sequencing technology,two transcriptome library of hemolymph was constructed. Unigene could be annotated to biological pathway by KEGG database to predict differential expression network of immune signaling pathway in C. sinensis. The results showed that 2 605 differentially expressed genes were found and a total of 893 genes annotated to 15 immune related pathways. This research could provide basis for exploring immune recognition and signal transduction pathway in C. sinensis.

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