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Position: Home > Articles > Isolation and Identification of Subtype H9N2 Avian Influenza Virus from Shaanxi Province and Sequence Analysis of It's HA、NP、NA Gene China Animal Health Inspection 2006,23 (1) 25-27+43

H9N2亚型禽流感病毒陕西分离株的鉴定及HA、NP、NA全序列测定

作  者:
曹维伟;陈杰;张燕霞;黄保续;洪军;陈德坤;李晓成
单  位:
农业部动物检疫所国家动物流行病学中心;西北农林科技大学动物科技学院
关键词:
H9N2亚型;禽流感病毒;HA;NP;NA;序列比较
摘  要:
从陕西地区疑似流感发病鸡分离到4株流感病毒,经国家流感中心鉴定均为A型流感病毒H9N2亚型。将其中1株A/Chicken/Shaanxi/3/2002(H9N2)的血凝素基因核蛋白基因和神经氨酸酶基因进行克隆和测序,与GenBank收录的其它流感H9N2亚型的相关基因进行比较,结果表明,A/Chicken/Shaanxi/3/2002(H9N2)的NA序列与A/Chicken/Shanghai/F/98(H9N2)的NA序列同源性较高,为98.9%,但其HA序列与香港人流感病毒A/HongKong/1073/99、A/HongKong/1074/99(H9N2)的HA序列同源性较低,为85.9%。另外,氨基酸进化树分析结果显示,A/Chicken/Shaanxi/3/2002(H9N2)与A/chicken/HongKong/CSW153/03(H9N2)的亲缘关系最近,两者形成独立的分支。
译  名:
Isolation and Identification of Subtype H9N2 Avian Influenza Virus from Shaanxi Province and Sequence Analysis of It's HA、NP、NA Gene
作  者:
Cao Weiwei1﹑2) Chen Jie2) Zhang Yanxia2) Huang Baoxu2) Hongjun2) Chen Dekun1) Li Xiaocheng2) (1,Animal Science Academy, Northwest Sci-tech University of Agriculture and Forestry, Yangling Shanxi 712100 2, China Epizootiology Centre, Animal Quarantine Institute of MOA Qingdao Shandong 266032)
关键词:
H9N2 subtype; avian influenza virus; HA; NP; NA; gene sequence
摘  要:
Four strains of avian influenza virus were isolated from sick chickens in different farms of Shaanxi province from May 1998 to May 2003.They were all identified as influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA?NP?NA gene of one isolate A/Chicken/Shaanxi/3/2002(H9N2) was cloned,sequenced and compared with sequences available in GenBank. The results indicated that the isolate A/Chicken/Shaanxi/3/2002(H9N2)possibly came from A/Chicken/Shanghai/F/98.

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