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Position: Home > Articles > The phylogenic analysis of two H9N2 subtype avian influenza virus isolates in Xiamen Animal Husbandry & Veterinary Medicine 2018 (12) 73-79

2株H9N2亚型禽流感病毒厦门分离株遗传演化分析

作  者：
蔡振鸿;陈琼;赵冉;孔繁德;程小文
单  位：
福建省厦门市动物卫生监督所;厦门出入境检验检疫局;福建省厦门市动物疫病预防控制中心;福建省厦门市思明区市政管理中心
关键词：
禽流感病毒;H9N2亚型;全基因组测定;分子遗传进化分析
摘  要：
对2015年活禽交易市场和养殖场的健康鸡群分离到的2株H9N2亚型禽流感病毒进行全基因组序列测定和遗传演化分析。结果显示:分离株的部分基因与1999年3株感染人的H9N2亚型同源性较低(86.3%～94.6%),与H7N9、H10N8、H10N6亚型同源性较高; A/chicken/Xiamen/10/2015分离株的NP基因与2013年感染人的H7N9毒株A/Shanghai/4664T/2013同源性高达99.9%,说明分离株的进化程度主要与时间相关,与宿主和地理位置关系不大。
译  名：
The phylogenic analysis of two H9N2 subtype avian influenza virus isolates in Xiamen
作  者：
CAI Zhenhong;CHEN Qiong;ZHAO Ran;KONG Fande;CHENG Xiaowen;Siming District Public Facilities Management Center of Xiamen City;Animal Disease Control and Prevention Center of Xiamen Ctiy;Xiamen Entry-exit Inspection and Quarantine Bureau;Xiamen Institute of Animal Health Supervision;
单  位：
CAI Zhenhong%CHEN Qiong%ZHAO Ran%KONG Fande%CHENG Xiaowen%Siming District Public Facilities Management Center of Xiamen City%Animal Disease Control and Prevention Center of Xiamen Ctiy%Xiamen Entry-exit Inspection and Quarantine Bureau%Xiamen Institute of Animal Health Supervision
关键词：
avian influenza virus;;H9N2;;complete genome sequencing;;phylogenic analysis
摘  要：
Two H9N2 influenza viruses were isolated from live chickens in Xiamen City in 2015.One was isolated from healthy chickens in a live poultry market and the other was isolated from healthy chickens in a poultry farm.For these isolates,their complete genomes were amplified,sequenced and analyzed.The results indicated that the two isolates were multi-origin reassortant viruses,highly homologous with the H9N2 isolates obtained in recent years,and some of the internal genes were also homologous with the H7N9,H10N8 and H10N6 viruses.Compared with human original isolates,the two isolates were lowly homologous with H9N2 isolates of 1999(86.3%-94.6%),but highly homologous with H10 N8 and H7N9 isolates of 2013.The NP gene of the A/chicken/Xiamen/10/2015 strain was 99.9% homologous with the H7N9 strain A/Shanghai/4664 T/2013.The degree of evolution of the isolates was mainly related to time,but little related with the host and geographical location.

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