当前位置: 首页 > 文章 > 用于有机磷农药残留快速检测的固定化小麦酯酶研究 食品科学 2003,24 (5) 122-126
Position: Home > Articles > Rapid Assay Study on Dichlocvos Residue by lmmobilized Wheaf Estecase Extract FOOD SCIENCE 2003,24 (5) 122-126

用于有机磷农药残留快速检测的固定化小麦酯酶研究

作  者:
许学勤;徐斐;华泽钊
单  位:
上海理工大学动力学院
关键词:
敌敌畏;有机磷农药;检测;小麦酯酶;离子交换树脂;酶的抑制;固定化酶
摘  要:
以大孔径强碱性阴离子交换树脂作为固定化载体,采用离子结合法,对植物粗酶液进行固定化。在室温及酶液对载体比例为4(ml):1(g)的条件下,对酶固定化的环境pH值、缓冲液浓度及固定化特性进行了研究。得到的固定化酶对10-7mol/L水平下敌敌畏的抑制仍有明显的响应,这一效果明显优于游离酶的抑制响应。
译  名:
Rapid Assay Study on Dichlocvos Residue by lmmobilized Wheaf Estecase Extract
作  者:
Xu Xueqin
关键词:
dichlorvos;organic phosphoric pesticide;detection;wheat esterase;ion exchanging resin;enzymeinhibition;immobilized enzyme
摘  要:
Extracted crude wheat esterase was immobilized by ion combination method. The carrier of the immobilized enzymewas a strong basic anion exchanging resin. The effects of pH value, concentration of enzyme extracting buffer and the esteraseactivity of enzyme solution on the immobilization, as well as the kinetic characteristics of the immobilization have been investigatedunder room temperature and a fixed ratio of enzyme solution to the carrier was established. The imobilized enzyme has beenremarkably inhibited even by the level of 10-7mol/L of dichlorvos. The inhibitory effect was superior to the result of free enzyme.

相似文章

计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊