中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 亮氨酸对秋繁时黄颡鱼精子活力的影响 华中农业大学学报 2019 (4) 71-76
Position: Home > Articles > 亮氨酸对秋繁时黄颡鱼精子活力的影响 Journal of Huazhong Agricultural University 2019 (4) 71-76

亮氨酸对秋繁时黄颡鱼精子活力的影响

作  者：
苏子豪;梅洁;田进松;何焱
关键词：
亮氨酸;黄颡鱼;秋繁;精子活力;受精率
摘  要：
在黄颡鱼(Pelteobagrus fulvidraco)精卵质量较差的秋繁早期,挑选体质量接近100 g的雄性黄颡鱼并分为3组,分别注射生理盐水(对照组)、100μmol/L亮氨酸(亮氨酸适量处理组)和1 000μmol/L亮氨酸(亮氨酸高浓度处理组),剂量均为200μL,研究亮氨酸对秋繁时黄颡鱼精子活力的影响.通过计算机辅助精子分析(CASA)系统检测精子活力,发现亮氨酸适量处理组的精子活力衰退速度减小,精子运动时间提高.将3组黄颡鱼精子用于人工繁殖,发现亮氨酸适量处理组受精率为34.9%±4.7%,相比对照组的15.9%±2.2%有显著性提高;亮氨酸高浓度处理组精子的受精率为16.4%±3.4%,与对照组相比无明显变化(P<0.05).结果表明适量的亮氨酸能提高黄颡鱼精子活力,但过量的亮氨酸并不能提高精子的繁殖能力.在黄颡鱼秋繁生产上可以适度添加亮氨酸以提高受精率.
作  者：
SU Zihao;MEI Jie;TIAN Jinsong;HE Yan;College of Fisheries,Huazhong Agricultural University;
关键词：
leucine;;yellow catfish;;autumn breeding;;sperm motility;;fertility
摘  要：
To study the effects of leucine on the sperm motility of yellow catfish(Pelteobagrus fulvidraco) during early stage of autumn breeding when the quality of sperms and eggs is lower than in summer breeding,males about 100 g were divided into three groups and injected with 200 μL physiological saline(control group),100 μmol/L leucine(moderate leucine treatment group) and 1 000 μmol/L leucine(high concentration leucine treatment group),respectively.It was found that sperm motility of the moderate leucine treatment group declined more slowly detected by the computer assisted sperm analysis(CASA) system.In artificial reproduction,the fertilization rate of the moderate leucine treatment group sperm was 34.91%±4.7%,significantly higher than that of the control group(15.9%±2.2%)(P<0.05),and the fertilization rate of the high concentration leucine treatment group sperm was 16.4%±3.4%,significantly lower than that of the moderate group(P<0.05),but not significant different from the control group(P>0.05).The results showed that moderate leucine could enhance the sperm motility of yellow catfish,but excessive leucine could not improve the sperm reproduction.So appropriate leucine can be added to improve the sperm reproduction of yellow catfish during the early stage of autumn breeding.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46