作 者：
冯志彬;薛钰;陈国忠;张娟;罗乐;程仕伟
关键词：
莫哈韦芽孢杆菌;壳聚糖酶;壳寡糖;分离鉴定;发酵条件优化
摘 要：
从烟台海岸带沙质土壤中分离筛选壳聚糖酶产生菌株,对其进行分类鉴定,优化其发酵条件,为酶法生产壳寡糖提供技术依据。通过透明圈法初步判断产酶能力,液体发酵复筛测定酶活力,筛选到酶活力达36.20 U/m L的菌株amy P216。通过菌体形态、菌落特征、生理生化及16S r DNA序列分析鉴定菌株amy P216为莫哈韦芽孢杆菌(Bacillus mojavensis)。通过单因素和正交试验确定最佳发酵条件为:胶体壳聚糖添加量20 g/L、酵母浸粉添加量12.5 g/L、吐温-80添加量1.0 g/L、初始发酵p H 6.0、发酵温度30℃。在最优条件下利用5 L自控发酵罐培养45 h壳聚糖酶活力可达到80.60 U/m L,较优化前(36.20 U/m L)提高了1.2倍。莫哈韦芽孢杆菌amy P216是一株壳聚糖酶活力较高的生产菌株,具有潜在的研究和应用价值。
译 名：
Isolation,Identification of Bacillus mojavensis amy P216 and Optimization of Its Fermentation Conditions for Enhanced Chitosanase Production
作 者：
FENG Zhibin;XUE Yu;CHEN Guozhong;ZHANG Juan;LUO Le;CHENG Shiwei;College of Life Science,Ludong University;College of Agriculture,Ludong University;
关键词：
Bacillus mojavensis;;chitosanase;;chitosan oligosaccharide;;isolation and identification;;optimization of fermentation conditions
摘 要：
Bacterial strains were isolated from coastal sandy soil collected in Yantai,Shandong province,and were initially screened to obtain 5 chitosanase-producing strains by transparent circle method.Further secondary screening provided a strain producing the highest chitosanase activity named amy P216.The strain amy P216 was identified as Bacillus mojavensisbased on morphological,physiological and biochemical characteristics and 16 S r DNA sequence analysis.The fermentation conditions of amy P216 for chitosanase production were optimized by one-factor-at-a-time and orthogonal array designs.The results showed that the maximum chitosanase activity of 80.60 U/m L,which was increased by 2.2 folds as compared to that before optimization(36.20 U/m L),was obtained when the fermentation was carried out at 30 ℃ for 45 h in a 5-L auto-controlled fermentor using optimized culture medium containing 20 g/L colloid chitosan,12.5 g/L yeast extract powder and 1.0 g/L Tween-80 at initial p H 6.0.Therefore,the strain amy P216 has potential application in the industrial production of chitosanase.
