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Position: Home > Articles > Development of A Colloidal Gold Strip for Rapid Detection of Listeria monocytogenes in Foods FOOD SCIENCE 2010,31 (4) 239-242

胶体金试纸快速检测食品中单增李斯特菌

作  者:
崔焕忠;张辉;王兴龙
单  位:
吉林农业大学动物科技学院;军事医学科学院军事兽医研究所
关键词:
单增李斯特菌;单克隆抗体;胶体金试纸;检测
摘  要:
制备单增李斯特菌(Lm)特异性单克隆抗体,研制胶体金试纸,快速检测食品中Lm。所研制的胶体金试纸具有较高的特异性,不与绵羊李斯特菌、英诺克李斯特菌同属菌及大肠杆菌、沙门氏菌、枯草杆菌、金黄色葡萄球菌、链球菌异属菌发生交叉反应;对Lm纯培养物检测的灵敏度为3.9×105CFU/mL;4℃保质期在120d以上;对自来水、牛奶、生肉制品、蔬菜、冷冻虾仁和面包模拟样品检测的灵敏度为3.9×105CFU/mL。该试纸具有快速、敏感、特异的优点,可用于食品中Lm的快速检测。
译  名:
Development of A Colloidal Gold Strip for Rapid Detection of Listeria monocytogenes in Foods
作  者:
CUI Huan-zhong1,ZHANG Hui1,WANG Xing-long2 (1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China ;2.Military Veterinary Institute,Academy of Military Medical Sciences of PLA,Changchun 130062,China)
关键词:
Listeria monocytogenes;monoclonal antibody;colloidal gold strip;detection
摘  要:
In the present study,a specific monoclonal antibody against Listeria monocytogenes (Lm) was prepared to develop a rapid immune colloidal gold strip for detecting Lm in foods.The developed colloidal gold strip had high specificity,no crossreactivity with L.ivanovii,L.innocua,Escherichia coli,Salmonella,Bacillus subtilis,Staphylococcus aureus and Streptococcus,a sensitivity of 3.9 × 105 CFU/mL in detecting Lm pure culture and simulated samples of tap water,milk,raw meat products,vegetables,frozen shrimp and bread,and more than 120-day expiration date at 4 ℃.The merits of rapidity,sensitivity,and specificity demonstrate a good applicability of this strip to the detection of Lm in foods.

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