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当前位置: 首页 > 文章 > 过表达天山雪莲SikCDPK1基因提高转基因烟草耐低温能力的机制初探 中国烟草学报 2016,22 (6) 98-103
Position: Home > Articles > Effect of over-expression of Saussurea involucrata Sik CDPK1 gene on improving cold tolerance in transgenic tobacco Acta Tabacaria Sinica 2016,22 (6) 98-103

过表达天山雪莲SikCDPK1基因提高转基因烟草耐低温能力的机制初探

作  者：
田晓涵;庞学兵;祝建波;朱新霞
单  位：
石河子大学生命科学学院农业生物技术重点实验室
关键词：
天山雪莲;SikCDPK1基因;低温;表达分析;转基因
摘  要：
钙依赖型蛋白激酶(calcium-dependent protein kinases,CDPKs)在植物参与非生物胁迫信号响应中发挥着重要作用。本研究利用实时荧光定量PCR技术分析了天山雪莲钙依赖性蛋白激酶基因Sik CDPK1在-4℃低温胁迫处理下的表达量,发现低温胁迫可以诱导天山雪莲内源Sik CDPK1基因表达,且在处理3 h表达量迅速积累达到最高值。将Sik CDPK1基因构建在由组成型CaMV35S强启动子控制的双子叶转化载体pCAMBIA2300上,利用农杆菌介导法转化烟草NC89,发现转基因烟草植株在低温胁迫后,耐低温能力明显增强;脯氨酸、可溶性蛋白含量及超氧化物歧化酶、过氧化物酶活性均高于非转基因烟草植株;丙二醛含量和相对电导率均低于非转基因烟草植株。上述研究结果表明,SikCDPK1基因可以通过积累渗透调节物质、增强抗氧化酶活性和维持细胞膜稳定性来提高植物的耐低温能力。
译  名：
Effect of over-expression of Saussurea involucrata Sik CDPK1 gene on improving cold tolerance in transgenic tobacco
作  者：
TIAN Xiaohan;PANG Xuebing;ZHU Jianbo;ZHU Xinxia;Key Laboratory of Agricultural Biotechnology,College of Life Science, Shihezi University;
关键词：
Sasussured involucrata Kar;;et Kir;;SikCDPK1;;low temperature;;expression analysis;;transgenosis
摘  要：
CDPKs(calcium-dependent protein kinases) play a very important role in abiotic stress response. Real-time PCR was used to analyze expression of SikCDPK1 gene to cold stress in Saussurea involucrata. Results showed that endogenous SikCDPK1 gene was induced by cold stress, and Sik CDPK1 expression reached the peak after 3 h. The SikCDPK1 gene was constructed on dicotyledonous transformation vector p CAMBIA2300, which was composed of a strong promoter of Ca MV35 S, and the gene was introduced into tobacco NC89 by Agrobacturium-mediated transformation method to identify the gene function. It turned out that after cold stress, cold resistance of transgenic tobacco plants was significantly enhanced, and the content of proline, soluble protein and the activities of POD and SOD in transgenic tobacco plants were higher than that of wild type, whereas the MDA content and electrolyte leakage were lower than that of wild type plants. These results indicated that SikCDPK1 gene could enhance the resistance of plants to cold stress by accumulating more osmotic adjustment substances, enhancing the activity of the antioxidant system and maintaining the stability of the cell membrane.

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