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当前位置: 首页 > 文章 > 机械搅拌生物反应器的CFD模拟及其在发酵生产乳酸中的应用 食品科学 2010,31 (7) 186-189
Position: Home > Articles > CFD Simulation of A Mechanically Stirred Bioreactor and Its Application in Lactic Acid Production FOOD SCIENCE 2010,31 (7) 186-189

机械搅拌生物反应器的CFD模拟及其在发酵生产乳酸中的应用

作  者：
吴学凤;姜绍通;张旻;刘模;庞锐
单  位：
合肥工业大学生物与食品工程学院
关键词：
CFD模拟;生物反应器;乳酸;米根霉
摘  要：
用计算流体力学(CFD)方法中的Mixsim模拟3L机械搅拌生物反应器中不同搅拌转速对搅拌流场的影响,结果显示Mixsim软件可以很好的模拟机械搅拌生物反应器工作过程中的流场变化。在高转速下,剪切速率较大,菌丝体98%以上为菌丝体小球,直径在0.1～0.3mm,菌体生长速度慢,产乳酸质量浓度较低;低转速下产生的剪切速率较小,菌体大部分为菌丝形态,产乳酸质量浓度较高,但是大量菌丝不利于后期发酵的进行。在400r/min下米根霉发酵产乳酸质量浓度最高,为106.7g/L,菌体80%以上为1.0～1.5mm左右的菌丝球,通过CFD模拟得到该条件下的剪切速率最大值为1.58m/s。
译  名：
CFD Simulation of A Mechanically Stirred Bioreactor and Its Application in Lactic Acid Production
作  者：
WU Xue-feng,JIANG Shao-tong,ZHANG Min,LIU Mo,PANG Rui(School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China)
关键词：
CFD simulation;bioreactor;lactic acid;Rhizopus oryzae
摘  要：
Mixsim,one of computational fluid dynamics(CFD) methods,was used to simulate a 3 L mechanically stirred bioreactor to study the effect of agitation speed on agitation current field.Along with this,effect of agitation speed on formation of mycelial pellets of Rhizopus oryzae and lactic acid production in the real bioreactor.Results showed that higher agitation speeds corresponding to higher shear stress rates resulted in the formation of more than 98% of mycelial pellets of 0.1-0.3 mm in diameter,with lower lactic acid production and biomass.In contrast,when agitation speed was controlled at lower levels and shear stress rate was also lower,the formation of mycelial pellets was inhibited and higher lactic acid production was achieved.However,the large amount of dispersed mycelia exhibited a negative impact on latter fermentation.A maximum lactic acid production of 106.7 g/L fermentation broth was achieved at the agitation of 400 r/min.Meanwhile,more than 80% of Rhizopus oryzae mycelia were formed into pellets of 1.0-1.5 mm in diameter.The biggest shear stress rate simulated by CFD was 1.58 m/s.

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