单 位：
贺州学院桂东特色资源开发与利用研究实验室
关键词：
马蹄皮;多酚;Folin-Ciocalteu试剂;分光光度法
摘 要：
根据GB/T8313-2008(茶叶中茶多酚的检测方法),使用Folin-Ciocalteu(FC)试剂和没食子酸标准品,采用分光光度法测定了马蹄皮中多酚的含量。从测定波长、试剂用量、稳定性、线性关系、重现性等方面,对GB/T8313-2008方法进行了调整。标准曲线的回归方程为y赞=0.012x-0.001(r=0.999 4),最佳测定条件为:0.20 mL 1 mol·L-1 FC试剂,0.15 mL 10%的Na2CO3,25℃反应80 min后于765 nm处测吸光值。使用调整后的方法测得马蹄皮多酚含量为4.86%,RSD为1.03%(n=6),平均回收率99.5%(RSD=0.269%,n=6)。该方法简便、快速、准确,是测定马蹄皮中多酚含量的可靠方法。
译 名：
Determination of Polyphenol in Water Chestnut Peel by Folin-Ciocalteu Method
作 者：
LUO Yang-he(Province Key Construction Laboratory of East Guangxi Characteristic Resources Development and Utilization Research,Hezhou University,Hezhou 542800,Guangxi,China)
关键词：
water chestnut peel;polyphenol;Folin-Ciocalteu reagent;spectrophotometry
摘 要：
According to GB/T8313-2008(determination of total polyphenols in tea),the total polyphenols(TP) content in water chestnut peel was analyzed by spectrophotometry with Folin-Ciocalteu reagent and standard of Gallic acid.The method of GB / T8313-2008 was improved and verified in the aspects of wavelength,reagent,stability,linearity and reproducibility,respectively.The regressive equation of standard curve was y=0.012x-0.001(r=0.999 4).The optimal determination conditions could be well calculated according to the absorption at 765 nm by reacting 0.20 mL 1 mol.L-1 Folin-Ciocalteu with 0.15 mL 10% Na2CO3 for 80 min at 25℃.In this method,the TP contents in water chestnut peel and RSD were 4.86% and 1.03%(n=6),respectively.The average recovery was 99.5%(RSD=0.269%,n=6).As a result,the improved method was convenient,fast,accurate and reliable for the determination of TP in water chestnut peel.
