中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 基于MHC-DAB基因内含子序列的3种虹鳟养殖群体遗传结构分析 中国农业科技导报 2013 (6) 135-141
Position: Home > Articles > Genetic Structure Analysis of Three Cultured Populations of Rainbow Trout(Oncorhynchus mykiss) Based on MHC-DAB Gene Intron Sequence Journal of Agricultural Science and Technology 2013 (6) 135-141

基于MHC-DAB基因内含子序列的3种虹鳟养殖群体遗传结构分析

作  者：
杨娟;刘山东;施海娜;胡丹丹;王建福;宋超;张久盘;刘哲
单  位：
甘肃农业大学动物科学技术学院;甘肃省渔业技术推广总站
关键词：
虹鳟(Oncorhynchus mykiss);遗传结构;遗传分化;MHC
摘  要：
虹鳟是当前全球性养殖品种,采用PCR-SSCP的方法,分析了甘肃金鳟、道氏虹鳟和美国金鳟三个虹鳟养殖群体各30个个体MHC-DAB基因第2内含子序列(308 bp)的遗传变异。108个阳性克隆经SSCP筛选及测序,共获得11种不同的核苷酸序列(Ⅰ~Ⅺ),单个个体最多存在4种等位基因。等位基因Ⅱ、Ⅲ、Ⅵ是三个群体共有的;Ⅳ仅存在于道氏虹鳟中;Ⅶ仅存在于甘肃金鳟中;Ⅸ、Ⅹ、Ⅺ仅存在于美国金鳟中。甘肃金鳟、道氏虹鳟、美国金鳟群体内核苷酸多态位点分别为17个、19个、22个,核苷酸多样性指数(Pi)分别为0.005、0.008、0.013,平均核苷酸位点差异数(K)分别为1.200、2.000、3.333。甘肃金鳟群体与道氏虹鳟群体间的遗传距离最小(0.231),与美国金鳟群体间的遗传距离最大(0.539),三个群体间的遗传分化均达到了种群的分化标准(D>0.05)。群体遗传分化系数G ST为0.097,表明变异主要来源于群体内个体间。
译  名：
Genetic Structure Analysis of Three Cultured Populations of Rainbow Trout(Oncorhynchus mykiss) Based on MHC-DAB Gene Intron Sequence
作  者：
YANG Juan;LIU Shan-dong;SHI Hai-na;HU Dan-dan;WANG Jian-fu;SONG Chao;ZHANG Jiu-pan;LIU Zhe;College of Animal Science and Technology,Gansu Agricultural University;Gansu Fisheries Technology Extension Station;
关键词：
Oncorhynchus mykiss;;genetic structure;;genetic differentiation;;MHC
摘  要：
Oncorhynchus mykiss was cultured all over the world,this paper analyzed the genetic variation of MHC-DAB intron 2 sequences( 308 bp) in Gansu golden trout,Donaldson rainbow trout and USA Golden trout using PCRSSCP.11 different nucleotide sequences( Ⅰ ~ Ⅺ) were detected from 108 positive clones,a maximum of 4 alleles were amplified from one single individual.Allele Ⅱ,Ⅲ and Ⅵ were presented in all the 3 populations.Ⅳ was only presented in Donaldson rainbow trout;Ⅶ only presented in Gansu golden trout;Ⅸ,Ⅹ and Ⅺ only presented in Golden trout.In Gansu golden trout,Donaldson rainbow trout and Golden trout,the nucleotide polymorphic sites were 17,19 and 22.The nucleotide diversities( Pi) were 0.005,0.008 and 0.013.The average numbers of nucleotide differences( K) were 1.200,2.000 and 3.333,respectively.The genetic distance of Gansu golden trout and Donaldson rainbow trout was the smallest( 0.231),while that of Gansu golden trout and Golden trout was the largest( 0.539).The genetic differentiation that compared any 2 of the 3 populations all up to the standard of population division( D > 0.05).The genetic differentiation index( G ST) of the 3 populations was 0.097,indicating that the variation mainly came from the inter-individual of the population.

相似文章

导出引用
计量
文章访问数: 17
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46