中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > β-羟丁酸对奶牛乳腺上皮细胞内乳脂肪合成及其相关基因相对表达量的影响 动物营养学报 2015,27 (1) 196-203
Position: Home > Articles > Effects of β-Hydroxybutyric Acid on Relative Expression Levels of Genes Related to Milk Fat Synthesis in Bovine Mammary Epithelial Cells Chinese Journal of Animal Nutrition 2015,27 (1) 196-203

β-羟丁酸对奶牛乳腺上皮细胞内乳脂肪合成及其相关基因相对表达量的影响

作  者：
常晨城;齐利枝;闫素梅;生冉;赵艳丽
单  位：
内蒙古农业大学动物科学学院
关键词：
β-羟丁酸;乳腺上皮细胞;乳脂肪合成相关基因;奶牛
摘  要：
本试验主要研究了不同浓度的β-羟丁酸(BHBA)对奶牛乳腺上皮细胞(BMECs)活力、甘油三酯(TAG)含量、脂滴形成以及乳脂肪合成相关基因转录水平的影响。将传至第3代的BMECs悬液(1×105个/孔)接种于细胞培养板上,每孔加入含10%胎牛血清(FBS)的DM EM/F12培养液,于37℃的5%二氧化碳(CO2)培养箱培养48 h。再将培养48 h的BMECs随机分配到6个组,各组向培养孔中加入含不同浓度BHBA的DMEM/F12培养液,培养液中的FBS用1 g/L无脂肪酸的牛血清白蛋白(BSA)代替,并使反应体系中BHBA的最终浓度分别为0(对照)、0.58、1.16、2.32、4.64和9.28 mmol/L。置于37℃的5%CO2培养箱继续培养48 h。试验结果显示:随着BHBA浓度的增加,BMECs活力[(相对增殖率(RGR)]呈显著的二次曲线增加(P=0.041),其中BMECs活力以0.58~4.64 mmol/L BHBA组较高,9.28 mmol/L BHBA组较低;低浓度(0.58~2.32 mmol/L)的BHBA可促进BMECs内脂滴的形成,而较高浓度(4.64~9.28 mmol/L)的BHBA对脂滴形成的促进作用减弱;BHBA与TAG含量及乳脂肪合成相关基因脂肪酸合成酶(FASN)、乙酰辅酶A羧化酶α(ACACA)、硬脂酰辅酶A去饱和酶(SCD)、脂肪酸结合蛋白3(FABP3)、过氧化物酶体增殖物激活受体γ(PPARG)和分化抗原簇36(CD36)的相对表达量均无显著的一次线性或二次曲线关系(P>0.05)。综上,BHBA对BM ECs活力的促进作用呈显著的二次曲线增加,即BHBA对BM ECs活力呈显著浓度依赖关系;BHBA对细胞内乳脂肪的合成有提高的趋势。
译  名：
Effects of β-Hydroxybutyric Acid on Relative Expression Levels of Genes Related to Milk Fat Synthesis in Bovine Mammary Epithelial Cells
作  者：
CHANG Chencheng;QI Lizhi;YAN Sumei;SHENG Ran;ZHAO Yanli;College of Animal Science,Inner Mongolia Agricultural University;
关键词：
β-hydroxybutyric acid;;mammary epithelial cells;;genes related to milk fat synthesis;;dairy cows
摘  要：
This study was conducted to determine the effects of β-hydroxybutyric acid( BHBA) on cell viability,triacylglycerol( TAG) content,lipid droplet formation and relative expression levels of genes related to milk fat synthesis in bovine mammary epithelial cells( BM ECs). The 3th passage cells were plated 1 × 105 cells / well in culture plates and DM EM / F12 medium containing 10% fetal bovine serum( FBS) was added to each well,then the plates were maintained in an incubator with 5% CO2 at 37 ℃. After 48 h,cells were randomly divided into 6 groups and cultured in DM EM / F12 medium containing different concentrations of BHBA,FBS in medium was replaced with 1 g / L fatty acid-free bovine serum albumin( BSA). The final concentrations of BHBA in reaction systems were 0( control),0.58,1.16,2.32,4.64,9.28 mmol / L,respectively.Continuous incubation in an incubator with 5% CO2 at 37 ℃ for 48 h. The results showed that BM ECs proliferation [relative growth rate( RGR) ] was significantly quadratic increased with BHBA concentration increasing( P = 0.041),and BMECs proliferation of 0.58 to 4.64 mmol/L BHAB groups was high,but 9.28 mmol/L BHBA group was low. BHBA at lowconcentration( 0.58 to 2.32 mmol / L) promoted lipid droplet formation,and the promoting effect of BHBA at high concentration( 4.64 to 9.28 mmol / L) was weakened. No significant linear and quadratic relationships were found between BHBA concentration and TAG content( P > 0. 05). No significant linear and quadratic relationships were found between BHBA concentration and relative expression levels of genes related to milk fat synthesis( fatty acid synthetase,acetyl-Co A carboxylase α,stearyl coenzyme A desaturase,fatty acid binding protein 3,peroxisome proliferator-activated receptor γ and cluster of differentiation 36)( P> 0.05). In conclusion,BM ECs proliferation significantly quadratic increase with BHBA concentration increasing,namely BM ECs proliferation has significant concentration-dependent relationship with BHBA concentration,and the milk fat synthesis has a improve trend by BHBA concentration.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46