当前位置: 首页 > 文章 > 利用反相液相色谱技术纯化梅花鹿胎盘肽的条件研究 食品科学 2009,30 (24) 327-330
Position: Home > Articles > Purification of Deer Placenta Peptide by Reversed-phase High Performance Liquid Chromatography FOOD SCIENCE 2009,30 (24) 327-330

利用反相液相色谱技术纯化梅花鹿胎盘肽的条件研究

作  者:
王淑琴;皮钰珍;雷斌
单  位:
沈阳农业大学食品学院
关键词:
反相液相色谱;梅花鹿胎盘肽;纯化
摘  要:
利用反相液相色谱技术对梅花鹿胎盘肽进行纯化,考察三氟乙酸(TFA)的加入量、有机相的起始浓度、流速和温度对纯化效果的影响,确定最佳分离纯化条件为:色谱柱HypersilODSC18(250mm×4.6mm,5μm),流动相A液0.05%TFA-乙睛,B液0.05%TFA-水,30min内10%A~50%A,90%B~50%B线性梯度洗脱,流速1.0ml/min,温度30℃,检测波长214nm。
译  名:
Purification of Deer Placenta Peptide by Reversed-phase High Performance Liquid Chromatography
作  者:
WANG Shu-qin,PI Yu-zhen,LEI Bin (College of Food Science, Shenyang Agricultural University, Shenyang 110161, China)
关键词:
reversed-phase high performance liquid chromatogram;deer placenta peptide;purification
摘  要:
Reversed-phase high performance liquid chromatography was used to purify deer placenta peptide in this paper. Major factors such as addition amount of trifluoroacetic acid, initial concentration of organic phase, flow rate and temperature on separation efficiency were investigated. The optimal purification condition was Hypersil ODS C18 (250 mm × 4.6 mm, 5 μm), 0.05% TFA/CAN as the mobile phase A, 0.05% TFA/water as the mobile phase B, linear gradient elution with 10%-50% mobile phase A and 90%-50% mobile phase B for 30 min, 1.0 ml/min of flow rate at 30 ℃ and detection wavelength at 214 nm.

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