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Position: Home > Articles > Liquid Chromatographic Determination of Flavonoids in Rosa multiflora Thunb.from Xinjiang FOOD SCIENCE 2010,31 (12) 156-158

反相液相色谱法测定新疆野蔷薇果中的黄酮类化合物

作  者:
迪丽努尔·马力克;毛居代·亚尔买买提;阿孜古丽·木阿赛力木
单  位:
新疆师范大学生命科学与化学学院
关键词:
反相液相色谱;新疆野蔷薇;黄酮;芦丁;槲皮素
摘  要:
为建立一种简单、快速测定野蔷薇果中黄酮类化合物含量的反相液相色谱方法。利用甲醇、异丙醇和水以一定比例1mL/min流速梯度洗脱,360nm波长检测,芦丁和槲皮素在4min内即可达到基线分离。经与标准品对照,野蔷薇果中含有芦丁和槲皮素,含量分别为17.7、94.7μg/gmd。芦丁和槲皮素回收率较好,分别为95.6%和97.3%;相对标准偏差均小于5%,方法重复性较好。本法可用于野蔷薇果中黄酮类化合物的快速分析检测。
译  名:
Liquid Chromatographic Determination of Flavonoids in Rosa multiflora Thunb.from Xinjiang
作  者:
DILINUR Malike,MAOJUDAI Yaermaimaiti,AZIGULI Muasailimu(College of Life Science and Chemistry,Xinjiang Normal University,rmqi 830054,China)
关键词:
flavonoids;liquid chromatography;quercetin;Rosa multiflora Thunb.;rutin
摘  要:
A simple and rapid liquid chromatographic(LC) method was developed for determining total flavonoids in Rosa multiflora Thunb from Xinjiang.A mixture of methanol,isopropanol and deionized water was used for the chromatographic separation at a flow rate of 1 mL/min.The detection wavelength was set at 360 nm.Baseline separation was achieved for rutin and quercetin within 4 min.The contents of rutin and quercetin in Rosa multiflora Thunb from Xinjiang were 17.7 μg/g and 94.7μg/g(calculated on the basis of dry weight).Mean recoveries for rutin and quercetin at the spike level of 100μg/mL were 95.6% and 97.3%,respectively,and the RSDs were both below 5%.The repeatability of the developed method was good.As a conclusion,it is applicable for rapid determination of flavonoid compounds in Rosa multiflora Thunb,like rutin and quercetin.

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