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响应面法优化金银花多糖超声提取工艺研究

作  者:
赵鹏;李稳宏;朱骤海;李冬;高蓉
单  位:
西北大学化工学院
关键词:
金银花;超声提取;多糖;工艺条件;响应面分析
摘  要:
为优化金银花多糖的超声提取工艺,在单因素试验的基础上,选取温度、时间以及液料比为自变量,多糖提取率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖提取率的影响。利用响应面分析方法,模拟得到二次多项式回归方程的预测模型,并确定金银花多糖提取工艺的最佳条件为温度75℃、时间43min、液料比23:1(ml/g)、提取两次。在此条件下,多糖提取率达到3.263%。超声法与传统的回流提取法相比,最大的特点是提取时间明显缩短。
译  名:
Optimization of Ultrasonic Extraction for Polysaccharides from Lonicera japonica
作  者:
ZHAO Peng,LI Wen-hong,ZHU Zhou-hai,LI Dong,GAO Rong(College of Chemistry Engineering, Northwest University, Xi'an 710069, China)
关键词:
Lonicera japonica;ultrasonic extraction;polysaccharide;process;response surface analysis
摘  要:
In order to optimize ultrasonic extraction of polysaccharides in Lonicera japonica, a mathematical regression model for predicting extraction yield of polysaccharides as a function of ratio of independent variables, including water/material ratio, extraction temperature and extraction time was established by fitting Box-Benhnken central composite design experimental results and interactive effects of the independent variables on extraction yield of polysaccharides were investigated by response surface methodology based on the analysis of single-factor experiments.The optimal protocol for polysaccharides extraction from Lonicera japonica was based on twice extraction for 43 min at 75 ℃ with a ratio of water to dry material of 23:1, which yielded an extraction yield of 3.26%.Results indicated that ultrasonic method is characteristic of time-saving compared with other traditional methods.

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