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Position: Home > Articles > Studies on the inspection method for silkworm pebrine by PCR and development of the diagnosis kit China Animal Health Inspection 2009,26 (10) 49-53

家蚕微粒子病PCR快速检测技术研究及诊断试剂盒的研制

作  者:
刘骏;钱科;沈中元
单  位:
中国农业科学院蚕业研究所;镇江出入境检验检疫局
关键词:
家蚕微粒子病;PCR;口岸检测
摘  要:
根据比对大量微孢子虫ssu rRNA序列的基础上自行设计了检测家蚕微粒子病的通用型引物。本试验确定了PCR反应体系和反应条件,并进行了相关病原检测试验,且对家蚕的不同材料进行了PCR检测,结果表明,本试验建立了有效、特异、敏感的检测家蚕微粒子病的PCR检测方法,为家蚕微粒子病在口岸快速检出,提供了有效的手段。
译  名:
Studies on the inspection method for silkworm pebrine by PCR and development of the diagnosis kit
作  者:
LIU Jun1, Qian Ke1, SHEN Zhong-yuan2 (1.Zhenjiang entry-exit Inspection and Quarantine Bureau, Jiangsu, Zhenjiang 212008; 2.The Sericultural Research Institute, Chinese Academy of Agricultural Sciences (SRICAAS), Jiangsu, Zhenjiang 212008)
关键词:
Pebrine disease; PCR; The imports and exports Quarantine
摘  要:
By comparing kinds of microsporidia ssurRNA, a general PCR primer for the pathogeny of pebrine disease was designed. It has been proved that the methods of PCR for the pathogeny of pebrine disease was a simple, sensitive, specific technique, and this study has supplied the way for the imports and exports quarantine for pebrine disease

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