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当前位置: 首页 > 文章 > 四川地区鸡异刺线虫核糖体转录间隔区(ITS1/2)序列的遗传变异分析 畜牧兽医学报 2016,47 (4) 796-804
Position: Home > Articles > Genetic Variation of Heterakis gallinarumin Sichuan based on the Nuclear Ribosomal DNA Internal Transcribed Spacer Regions(ITS1/2) Chinese Journal of Animal and Veterinary Sciences 2016,47 (4) 796-804

四川地区鸡异刺线虫核糖体转录间隔区(ITS1/2)序列的遗传变异分析

作  者：
古小彬;朱俊扬;王保健;郑晶;杨光友
单  位：
四川农业大学动物医学院;四川农业大学动物科技学院;四川省绵阳市动物疫病预防控制中心四川农业大学西昌学院
关键词：
鸡异刺线虫;四川;ITS1/2序列;遗传多样性
摘  要：
探讨四川地区鸡异刺线虫(Heterakis gallinarum)的遗传变异情况,为该地区鸡异刺线虫病的流行特征及防控提供基础资料。采用PCR扩增四川7个地区共59个鸡源鸡异刺线虫核糖体ITS1-5.8S-ITS2片段并测序,所得序列剪切5.8S序列后,得到ITS1/2序列,并分析ITS1/2序列的遗传多样性;同时利用GenBank中已公布的异刺属(Heterakis)ITS1/2序列信息,进行系统发育研究。结果如下:59条鸡异刺线虫ITS1/2序列相似性较高(98.9%~100.0%),ITS1序列间变异较ITS2大;59条序列有20个变异位点和14个单倍型(H1~H14);四川种群具有丰富的单倍型多样性(Hd=0.532)和较低的核苷酸多样性(π=0.000 94);种群间基因交流频繁(Nm=31.72),群体遗传分化不明显(Fst=0.007 82),以群体内变异为主(AMOVA分析值=99.22%);从单倍型网络图及进化树(MP和ML)分析来看,7个地理种群未形成显著的地理种群结构,但四川整体种群曾经历过种群扩张(Tajima’s D=-2.553 31,Fu’s Fs=-10.564;P<0.05);进化树(MP和ML)能有效区分鸡异刺线虫与属内其他虫种。四川7个地区鸡的鸡异刺线虫基因交流频繁,遗传多样性较低。
译  名：
Genetic Variation of Heterakis gallinarumin Sichuan based on the Nuclear Ribosomal DNA Internal Transcribed Spacer Regions(ITS1/2)
作  者：
GU Xiao-bin;ZHU Jun-yang;WANG Bao-jian;ZHENG Jing;YANG Guang-you;WANG Tao;LAI Wei-min;College of Veterinary Medicine,Sichuan Agricultural University;Sichuan Entry-exit Inspection and Quarantine Bureau;
关键词：
Heterakis gallinarum;;Sichuan;;ITS1/2 sequences;;genetic variation
摘  要：
The aim of the present study was to analyze the characteristic of genetic variability in Heterakis gallinarum populations from Sichuan province,and provide a foundation for understanding the epidemiology and assist in the control of heterakiasis.The nuclear ribosomal DNA internal transcribed spacer region(ITS1-5.8S-ITS2)of 59 H.gallinarumisolates from chickens belonged to 7different geographical regions were amplified using the polymerase chain reaction(PCR)and then sequenced.Sequences unit containing ITS1 and ITS2(excepting 5.8S)were used to determine the genetic variability and genetic structure of H.gallinarumin Sichuan province,China.Meanwhile,the relationships of this nematode with selected members of genus Heterakis were assessed by phylogenetic analysis of ITS1/2sequence datasets by maximum parsimony(MP)and maximum likelihood(ML).Results showed that 59 H.gallinarumITS1/2sequencesidentity ranged from 98.9% to 100.0%,and ITS1 sequences displayed greater variability than ITS2.Fifty-nine sequences contained 20 variable sites,and were classified into 14haplotypes(H1-H14).The overall Sichuan population showed high haplotype diversity(Hd = 0.532)and low nucleotide diversity(π=0.000 94).Meanwhile,a frequency of gene flow(Nm=31.72),a low degree of genetic differentiation(Fst= 0.007 82),and an overwhelming genetic variability(99.22%)were observed within the overall Sichuan population.Phylogenetic trees(MP and ML)and haplotype network were both revealed that 7different geographical regions did not form significant geographical populations.The different species within genus Heterakis were well distinguished in phylogenetic trees(MP and ML).Our results indicate that there is a high gene flow and low degree of genetic diversity across H.gallinarumpopulation.

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