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当前位置: 首页 > 文章 > 基于线粒体12S基因分析四川地区鸡异刺线虫的种群遗传多态性 畜牧兽医学报 2015,46 (12) 2264-2272
Position: Home > Articles > Population Genetic Polymorphisms of Heterakis gallinarum in Sichuan Base on Mitochondrial 12S Gene Chinese Journal of Animal and Veterinary Sciences 2015,46 (12) 2264-2272

基于线粒体12S基因分析四川地区鸡异刺线虫的种群遗传多态性

作  者：
王保健;朱俊扬;简克灵;杨光友;汪涛;赖为民;赵习彬;廖晓霞;古小彬
单  位：
四川农业大学动物医学院
关键词：
鸡异刺线虫;12S;遗传多态性;四川
摘  要：
为探讨四川地区鸡异刺线虫的遗传变异特点和种群遗传结构特征,利用PCR技术扩增了四川7个地区共58株鸡源鸡异刺线虫分离株的线粒体12S基因全序列,经测序后分析其遗传多样性。结果显示,58条鸡异刺线虫12S基因全序列均为699bp,序列中共有38个变异位点和34个单倍型(HS1-HS34);单倍型多样性(Hd)和核苷酸多样性(π)分别为0.822和0.003 95。进一步分析表明,7个地理种群遗传分化不明显(Fst=0.007 87),种群间基因交流较频繁(Nm=31.52);分子方差分析(AMOVA)表明,四川地区鸡异刺线虫的遗传分化主要来自于种群内部(99.21%),种群间遗传变异水平较低(0.79%);单倍型网络图和NJ系统发育树显示,鸡异刺线虫7个地理种群的34个单倍型散在分布于不同种群内,分布格局较为混杂,未形成明显的地理分布格局。结果表明,四川地区的鸡异刺线虫遗传多样性较低,遗传分化不明显,还未形成显著的地理遗传结构。
译  名：
Population Genetic Polymorphisms of Heterakis gallinarum in Sichuan Base on Mitochondrial 12S Gene
作  者：
WANG Bao-jian;ZHU Jun-yang;JIAN Ke-ling;YANG Guang-you;WANG Tao;LAI Wei-min;ZHAO Xi-bin;LIAO Xiao-xia;GU Xiao-bin;College of Veterinary Medicine,Sichuan Agricultural University;
关键词：
Heterakis gallinarum;;12Sgene;;genetic polymorphisms;;Sichuan
摘  要：
To study the population genetic diversity and genetic structure of Heterakis gallinarum population in Sichuan,the complete sequence of mitochondrial 12 Sgene of 58 H.gallinarumisolates from 7different geographical regions in Sichuan were amplified and analyzed the genetic diversity.The results showed that the complete lengths of mitochondrial 12 Sgene sequenced from58 H.gallinarumisolates in this study were all 699 bp.These sequences contained 38 variable sites and were classified into 34haplotypes(HS1-HS34).The values of haplotypes diversity(Hd)and nucleotide diversity(π)were 0.822 and 0.003 95,respectively.Genetic diversity with Fst and Nm furtherly showed that there were no significant genetic differentiation and frequent gene flow among the seven populations(Fst=0.007 87,Nm=31.52).An analysis of molecular variance(AMOVA)estimated that 99.21% of the genetic variation was partitioned within the population,and only 0.79% occurred among populations.NJ phylogenetic tree and haplotype network were both revealed that 34 haplotypes from 7different geographical regions dispersed different geographical populations,and haplotypes did not cluster according to their geographical location.The results indicated that the H.gallinarumpopulation in Sichuan had a low level of genetic diversityand no significant genetic differentiation,it have not formed significant geographical genetic structure.

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