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当前位置: 首页 > 文章 > 禽多杀性巴氏杆菌不同培养方法制备的种子液对高密度发酵培养菌数的影响 动物医学进展 2015 (8) 120-123
Position: Home > Articles > Effects of Preparations of Seed Liquid with Different Methods on Number of Avian Pasteurella multocide by High Density Fermentation Culture Progress in Veterinary Medicine 2015 (8) 120-123

禽多杀性巴氏杆菌不同培养方法制备的种子液对高密度发酵培养菌数的影响

作  者：
吴信明;王艳;孙翠平;孟海滨;程立坤;韩文瑜;沈志强
单  位：
吉林大学动物医学学院;山东省滨州畜牧兽医研究院;山东绿都生物科技有限公司
关键词：
禽多杀性巴氏杆菌;扁瓶固体培养法;摇床液体培养法;发酵罐培养法;种子;高密度发酵
摘  要：
本试验用扁瓶固体培养法、摇床液体培养法和10L发酵罐培养法制备禽多杀性巴氏杆菌种子液,采用细菌比浊法和活菌计数法,计算高密度发酵菌液浓度,比较3种方法制备的种子液对禽多杀性巴氏杆菌抗原生产的影响。经过3批次试验,扁瓶固体培养法制备的种子液经高密度发酵培养后测得的活菌数为1.56×1010~1.65×1010 CFU/mL,比浊计数为2.10×1010~2.59×1010 CFU/mL;而摇床液体培养法制备的种子液经高密度发酵培养后测得的活菌数为1.54×1010~1.74×1010 CFU/mL,比浊计数为2.10×1010~2.45×1010 CFU/mL。发酵罐培养制备的禽多杀性巴氏杆菌种子接种200L发酵罐进行高密度发酵培养后测得的活菌数可达1.85×1010~2.05×1010 CFU/mL,比浊计数为2.45×1010~2.80×1010 CFU/mL。结果表明,10L发酵罐培养制备的种子液优于扁瓶固体培养法和摇床液体培养法制备的种子液,而后两者无明显差别;从3批次平均值看,前者比后两者活菌计数结果提高约21.60%,比浊计数结果提高约15.52%。说明采用10L发酵罐培养制备的禽多杀性巴氏杆菌种子进行高密度发酵培养可显著提高菌液浓度,增加单位抗原含量(P<0.01)。
译  名：
Effects of Preparations of Seed Liquid with Different Methods on Number of Avian Pasteurella multocide by High Density Fermentation Culture
作  者：
WU Xin-ming;WANG Yan;SUN Cui-ping;MENG Hai-bin;CHENG Li-kun;HAN Wen-yu;SHEN Zhi-qiang;Shandong Binzhou Animal Science & Veterinary Medicine Academy;College of Veterinary Medicine,Jinlin University;Shandong Lvdu Bio-industry Company,LTD.;
关键词：
avian Pasteurella multocide;;solid surface culture method;;liquor culturing on the shaker method;;liquor high density ferment culture method;;seed;;high density fermentation
摘  要：
The liquid seeds of avian Pasteurella multocide were cultivated with the solid culture in flat bottles,and the liquid culture on the shaking table and the 10-liter fermentation tank,respectively.Then the avian Pasteurella multocide antigen was prepared with high density fermentation in 200-liter fermentation tank by using the bacterial seeds.The bacterial density was evaluated by the viable cell count method and the turbidimetric method to compare the influences of bacterial seeds on the antigen production.After three batch tests,the viable cell count results showed that the amounts of living bacterial reached 1.56×1010-1.65×1010 CFU/mL,1.54×1010-1.74×1010 CFU/mL and 1.85×1010-2.05×1010 CFU/mL,respectively,with the seeds cultivated by the solid culture in flat bottles,the liquid culture on the shaking table and the 10-liter fermentation tank.The turbidimetry results showed that the amount of bacteria reached2.10×1010-2.59×1010 CFU/mL,2.10×1010-2.45×1010 CFU/mL and 2.45×1010-2.80×1010 CFU/mL,respectively.The conclusion:the seeds prepared with the 10-liter fermentation tank were superior to that with other two methods,and thereafter improved the quality of unit antigen significantly(P<0.01).

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